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Merck
CN

114545

Thymol Blue

ACS reagent

Synonym(s):

Thymolsulfonphthalein

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About This Item

Empirical Formula (Hill Notation):
C27H30O5S
CAS Number:
76-61-9
Molecular Weight:
466.59
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24847181
EC Number:
200-973-3
Beilstein/REAXYS Number:
368036
MDL number:
MFCD00005869

Key Documents

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InChI key

PRZSXZWFJHEZBJ-UHFFFAOYSA-N

InChI

1S/C27H30O5S/c1-15(2)19-13-22(17(5)11-24(19)28)27(21-9-7-8-10-26(21)33(30,31)32-27)23-14-20(16(3)4)25(29)12-18(23)6/h7-16,28-29H,1-6H3

SMILES string

CC(C)c1cc(c(C)cc1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C(C)C)c(O)cc4C

grade

ACS reagent

form

powder

clarity of soln

passes test

technique(s)

titration: suitable

visual transition interval

1.2-2.8, red to yellow(acid range), 1.2-2.8, red to yellow, 8.0-9.2, yellow to blue(alkaline range), 8.0-9.2, yellow to blue

mp

221-224 °C (dec.) (lit.)

solubility

ethanol: 0.1%, clear

density

0.979 g/cm3

λmax

376 nm (2nd), 594 nm

ε (extinction coefficient)

≥12000 at 298-302 nm in 0.1 M NaOH at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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General description

Thymol Blue is a sulfonephthalein dye. It is also known as thymolsulfonphthalein.

Application

Thymol Blue has been used for the in situ spectrophotometric pH measurement.

Biochem/physiol Actions

Thymol Blue is a pH indicator, which is yellow/orange at low pH and blue in color at high pH.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000517605
0000517605
0000496462
0000496462
0000448123

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Global stability for convection induced by absorption of radiation.
Straughan B
Dynamics of atmospheres and oceans, 35, 351-361 (2002)
Douglas R Macfarlane et al.
Chemical communications (Cambridge, England), 46(41), 7703-7705 (2010-09-28)
We describe hydrated ionic liquids which have an intrinsic proton buffering action and hence an ability to control proton activity in an ionic medium.
N Takai et al.
Artificial organs, 15(2), 86-89 (1991-04-01)
An optical fiber sensor utilizing Thymol blue and an ion-exchange resin complex in a cellulose acetate membrane was developed. By monitoring several different chromophores of Thymol blue, the sensor could measure the pH of the solution from 1.0 to 12.0
S Passamonti et al.
Biochimica et biophysica acta, 943(2), 119-125 (1988-08-18)
Sulfobromophthalein electrogenic uptake into rat liver plasma membrane vesicles was shown to admit only the quinoid, trivalent anion. The minimum requirement for this electrogenic process has been investigated in rat liver plasma membrane vesicles by using Thymol blue, a pH-indicator
N Gayathri et al.
Journal of AOAC International, 84(4), 1065-1069 (2001-08-15)
A simple and sensitive spectrophotometric method was developed for the determination of trace amounts of sulfur dioxide. The method is based on the reaction of SO2 with a known excess of ICI as the oxidant. The unreacted ICI iodinates thymol
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