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Merck
CN

17153

LS Differential Agar

NutriSelect® Plus, suitable for microbiology

Synonym(s):

Lactobacillus Streptococcus Differential Agar

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171606

Key Documents

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Product Name

LS Differential Agar, NutriSelect® Plus, suitable for microbiology

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
beef extract, 5 g/L
casein enzymic hydrolysate, 10 g/L
L-cysteine hydrochloride, 0.3 g/L
dextrose, 20 g/L
papaic digest of soybean meal, 5 g/L
sodium chloride, 5 g/L
yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

6.1±0.2 (25 °C)

application(s)

bioburden testing
environmental
food and beverages
microbiology

suitability

nonselective and differential for Lactobacillus spp.
nonselective and differential for Streptococcus spp.

Quality Level

200

Related Categories

Application

For the maximum growth and differentiation of Lactobacilli and Streptococci on the basis of colonial morphology, T.T.C. reduction and casein reaction.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Suspend 65.3 g in 1 litre distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and add the following sterile solutions previously kept warm at 50°C just prior to use; (1) 100 ml of 10% (w/v) aqueous solution of antibiotic free skim milk powder (Cat. No. No. 70166) sterilized at 121°C for 5 minutes.(2) 10 ml of TTC Solution (Cat. No. 17779). Mix well and pour into sterile petri plates.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCL3699
BCCK7840
BCCG3238
BCCB5483
BCCB0217

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Articles

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Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques

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Microbiological Media & Detection

Cost-effective molecular biology method for lactobacilli detection is presented as an alternative to PCR-based methods.

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链球菌 — 检测、鉴定、分化和培养技术概述

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