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Merck
CN

17202

Phosphate buffer

NutriSelect® Plus, APHA, final pH 7.2±0.2 (25 °C), powder, pkg of 500 g

Synonym(s):

Buffered Phosphate diluent, PBS, Phosphate Buffered Saline

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About This Item

UNSPSC Code:
41171610
NACRES:
NA.85
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Agency

APHA
according to GB 4789.10-2016
according to GB 4789.15-2016

Quality Level

description

APHA, pH 7.2

sterility

non-sterile

form

powder

composition

monopotassium phosphate, 26.22 g/L
sodium carbonate, 7.78 g/L

packaging

pkg of 500 g

manufacturer/tradename

NutriSelect® Plus

final pH

7.2±0.2 (25 °C)

application(s)

agriculture
environmental
food and beverages
microbiology
sample preparation
veterinary
water monitoring

General description

Phosphate Buffer, pH 7.2, is prepared according to APHA guidelines and is primarily used for diluting test samples. It is specified for microbiological methods involving the dilution of water, dairy products, and food samples. Additionally, Phosphate Buffer can be used with the addition of magnesium chloride, as recommended by APHA and FDA. This medium is also referred to as Butterfield’s Buffered Phosphate Diluent when used without magnesium chloride. Phosphate Buffer is preferred over unbuffered water as it helps standardize the pH variations commonly observed in distilled water from different sources.

Application

Phosphate Buffer is a microbiological culture medium designed for the preparation of dilutions and blanks for the examination of water, dairy products, foods, eating utensils, and other specimens in food and beverage industries.

Features and Benefits

NutriSelect® Plus are powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

Preparation Note

Dissolve 34 g in 1 litre distilled water. Dispense and sterilize by autoclaving at 121°C for 15 minutes. The pH, after autoclaving and cooling to room temperature should be 7.2 ± 0.2.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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M M Mahoney et al.
The European journal of neuroscience, 30(8), 1537-1543 (2009-10-09)
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Umut Gazi et al.
The Journal of biological chemistry, 286(10), 7822-7829 (2011-01-06)
The mannose receptor (MR) is an endocytic type I membrane molecule with a broad ligand specificity that is involved in both hemostasis and pathogen recognition. Membrane-anchored MR is cleaved by a metalloproteinase into functional soluble MR (sMR) composed of the

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