17274
Thermoacidurans Agar
NutriSelect® Plus, according to APHA, powder, nonselective for Bacillus coagulans, pkg of 500 g, suitable for microbiology
Synonym(s):
acidified proteose-peptone agar
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About This Item
NACRES:
NA.74
UNSPSC Code:
41171606
Manufacturer/tradename:
NutriSelect® Plus
Agency:
according to APHA
Form:
powder
Suitability:
nonselective for Bacillus coagulans
Application(s):
bioburden testing
food and beverages, microbiology
food and beverages, microbiology
Quality Segment
agency
according to APHA
sterility
non-sterile
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 20 g/L , dextrose, 5 g/L , dipotassium phosphate, 4 g/L , manganese sulfate, 0.2 g/L , proteose peptone, 5 g/L , yeast extract, 5 g/L
packaging
pkg of 500 g
manufacturer/tradename
NutriSelect® Plus
technique(s)
microbiological culture: suitable
final pH
5.0±0.2 (25 °C)
application(s)
bioburden testing
food and beverages, microbiology
suitability
nonselective for Bacillus coagulans
General description
Thermoacidurans Agar is a selective growth medium. It is recommended for culturing and isolating Bacillus coagulans (Bacillus thermoacidurans), a facultative thermophile. This bacterium can thrive in temperatures ranging from 20 to 55°C and at pH levels between 5.0 and 7.0. Thermoacidurans Agar contains proteose peptone and yeast extract, which provide essential nitrogenous compounds, vitamin B complex, and other vital nutrients necessary for bacterial growth. Additionally, dipotassium phosphate is incorporated to buffer the medium, ensuring stable pH levels during incubation. Dextrose is a primary energy source that promotes the development of B. coagulans.
Application
Thermoacidurans Agar is specifically utilized for the isolation, cultivation, and enumeration of Bacillus coagulans (Bacillus thermoacidurans) from canned food products, as they are known to cause flat-sour spoilage in low-acid foods packed in hermetically sealed containers. B. coagulans will form large, cream to white colonies.
Features and Benefits
NutriSelect® Plus is a powdered culture media offering:
- Quality control with growth promotion test according to industry standards
- Quality assurance acc. to ISO 9001
- Cost-efficient powdered media
Preparation Note
Suspend 39.2 g in 1 litre distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour into sterile petri plates.
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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