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Merck
CN

17779

2,3,5-Triphenyl-tetrazolium chloride solution

suitable for microbiology, Filter sterilized solution that is recommended for the detection of microbial growth based on reduction of TTC

Synonym(s):

TTC solution, TTC supplement, TTC solution, TTC supplement

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About This Item

Empirical Formula (Hill Notation):
C19H15ClN4
CAS Number:
298-96-4
Molecular Weight:
334.80
Beilstein:
3923356
MDL number:
MFCD00011963
UNSPSC Code:
41171614
PubChem Substance ID:
329751567
NACRES:
NA.85

Key Documents

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Quality Level

100

sterility

sterile (Filtered and Aseptic Handled)

form

liquid

shelf life

limited shelf life, expiry date on the label

mol wt

334.8 g/mol

application(s)

agriculture
environmental
food and beverages
microbiology

storage temp.

2-8°C

suitability

bacteria

SMILES string

[Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4

InChI

1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1

InChI key

PKDBCJSWQUOKDO-UHFFFAOYSA-M

Related Categories

General description

2,3,5-Triphenyl-tetrazolium chloride (TTC) is a dye largely used for the easy enumeration of microbial colonies in solid culture media. This dye is colorless in the oxidized form and changes to red when reduced by microorganisms, due to the formation of formazan. Live microorganisms, for example, E. coli and other coliforms reduce TTC by enzymatic action, forming red color formazan that is kept inside granules in the cells. TTC is highly recommended in testing pasteurized milk because there is a high percentage of microorganisms unable to reduce TTC in pasteurized milk, which cannot be detected by laboratory procedures. Higher concentrations of TTC may have an inhibitory effect on the growth of Gram-positive organisms.

Application

2,3,5-Triphenyl-tetrazolium chloride (TTC) solution is a redox indicator that can help indicate cellular respiration and identify viable and non-viable cells as well as identify microorganisms capable of reduction of TTC on solid culture media

Other Notes

(per vial)
2,3,5-Triphenyl tetrazolium chloride: 0.1 g, distilled water: 10 ml

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN4992
BCCM1108
BCCL1577
BCCJ6782
BCCH0596

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Itziar Perez-Terol et al.
Scientific reports, 9(1), 20017-20017 (2019-12-29)
Delayed enhancement cardiovascular magnetic resonance (MR) is the gold-standard for non-invasive assessment after myocardial infarction (MI). MR microscopy (MRM) provides a level of detail comparable to the macro objective of light microscopy. We used MRM and correlative histopathology to identify
Han-Dong Li et al.
Journal of neuroinflammation, 14(1), 151-151 (2017-07-30)
Cerebral ischemia is a leading cause of death and disability with limited treatment options. Although inflammatory and immune responses participate in ischemic brain injury, the molecular regulators of neuroinflammation after ischemia remain to be defined. Translocator protein 18 kDa (TSPO) mainly
Keiichi Hishikari et al.
Cardiovascular research, 81(1), 123-132 (2008-09-23)
Increased expression of several subtypes of prostaglandin E(2) receptors (EP1-4) has recently been described in clinical and experimental myocardial ischaemia/reperfusion (I/R) injury. However, their pathophysiological significance in I/R remains obscure. Thus, we determined whether the activation of the prostanoid receptor
Neel R Sodha et al.
European journal of cardio-thoracic surgery : official journal of the European Association for Cardio-thoracic Surgery, 33(5), 906-913 (2008-03-04)
Ischemia-reperfusion (I/R) injury, often encountered clinically, results in myocardial apoptosis and necrosis. Hydrogen sulfide (H(2)S) is produced endogenously in response to ischemia and thought to be cardioprotective, although its mechanism of action is not fully known. This study investigates cardioprotection
Linfeng Qian et al.
Aging, 11(15), 5368-5388 (2019-08-14)
This study is intended to figure out the function of microRNA-218 (miR-218) together with microphthalmia-associated transcription factor (MITF) on the cardiac fibrosis and cardiac function impairment in rat models of myocardial infarction (MI). The rats with MI exhibited cardiac function
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Articles

Vibrio Detection

Vibrios are motile, curved or comma-shaped bacilli and have a single polar flagella with sheet proteins. They are often found in open water, freshwater and saltwater.

弧菌检测

大多数弧菌采用葡萄糖等简单的 C 源作为能源，需要2-4％的NaCl或其他盐以及海水中存在的微量元素，即可实现最佳生长。有些物种可以使用多种能源。

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