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Merck
CN

18137

Ponceau 4R

analytical standard

Synonym(s):

New Coccine, Acid Red 18, Ponceau 4 R

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About This Item

Empirical Formula (Hill Notation):
C20H11N2Na3O10S3
CAS Number:
2611-82-7
Molecular Weight:
604.47
UNSPSC Code:
12164500
NACRES:
NA.24
E Number:
E124
PubChem Substance ID:
329751631
EC Number:
220-036-2
MDL number:
MFCD00004084
Colour Index Number:
16255
Beilstein/REAXYS Number:
4122340

Key Documents

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Product Name

Ponceau 4R, analytical standard

InChI key

SWGJCIMEBVHMTA-ZRUFZDNISA-K

InChI

1S/C20H14N2O10S3.3Na/c23-16-7-5-11-9-12(33(24,25)26)10-18(35(30,31)32)19(11)20(16)22-21-15-6-8-17(34(27,28)29)14-4-2-1-3-13(14)15;;;/h1-10,23H,(H,24,25,26)(H,27,28,29)(H,30,31,32);;;/q;3*+1/p-3/b22-21+;;;

SMILES string

[Na+].[Na+].[Na+].Oc1ccc2cc(cc(c2c1\N=N\c3ccc(c4ccccc34)S([O-])(=O)=O)S([O-])(=O)=O)S([O-])(=O)=O

grade

analytical standard

assay

≥98.0% (HPLC)

shelf life

limited shelf life, expiry date on the label

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

application(s)

cleaning products
cosmetics
food and beverages
personal care

format

neat

Quality Level

100

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Application

The analytical standards can also be used as follows:
  • Spectrophotometric quantitative estimation of Brilliant Blue, Sunset Yellow, Ponceau 4R, and Tartrazin from juice samples, using a transparent polymer matrix for extraction
  • Liquid-liquid microextraction of five synthetic food dyes from different food samples for their determination by high-performance liquid chromatography with a variable wavelength ultraviolet detector (HPLC-VWUVD)
  • Simultaneous analysis of five water-soluble synthetic colorants in different food samples by on-plate solid-phase extraction and thin-layer chromatography (TLC) with backlight-assisted detection
  • Multi-residue determination of 11 synthetic color food additives in high protein and fat content food products by HPLC combined with a diode array detector (DAD) or a tandem mass spectrometer (MS/MS)
  • Extraction of eight synthetic food dyes from 30 food samples of six types using an NH2-aminopropyl-modified silica SPE cartridge for their quantification by RP-HPLC coupled with UV detector

General description

Ponceau 4R is a sulfonated azo dye, used as a food colorant to give red color to different food products, such as alcoholic beverages, soft drinks, bakery products, candies, syrups, preserved fruits, etc. An acceptable daily intake (ADI) of 0.4 to 0.7 mg (kg body weight)-1 was set for Ponceau 4R by the European Food Safety Authority (EFSA) in 2009.

Other Notes

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM9882
BCCJ7954
BCCG7295
BCBZ3098
BCBV0621

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Genotoxicity of Synthetic Food Colorants
Journal of Food Science and Engineering, 1, 128-134 (2011)
Encyclopedia of Food Safety, 640-640 (2013)
Analysis of methanol-ethanol mixtures from falsified beverages using a dual biosensors amperometric system based on alcohol dehydrogenase and alcohol oxidase.
Bucur, Bogdan, Gabriel Lucian Radu, and Constantin Nicolae Toader
European Food Research and Technology, 226, 1335-1342 (2008)
A comprehensive study of the presence of some food additives in non-alcoholic beverages in Republic of Macedonia from the period 2008-2012.
Kostik, Vesna.
Journal of Hygienic Engineering and Design, 7, 123-131 (2014)
Yunfei Xie et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 96, 600-604 (2012-08-08)
Ponceau 4R is used as a coloring agent in many different products, such as food, drinks, medicines, cosmetics and tobacco. However, ponceau 4R also shows carcinogenic, teratogenic and mutagenic behavior in high doses. In this work, standard Raman, theoretical Raman
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