Skip to Content
Merck
CN

198099

Methyl violet 2B

certified by the BSC

Synonym(s):

Basic Violet 1

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

CAS Number:
8004-87-3
NACRES:
NA.47
PubChem Substance ID:
24851861
UNSPSC Code:
12171500
MDL number:
MFCD00012568
Beilstein/REAXYS Number:
8464808

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

InChI

1S/C24H27N3.ClH/c1-25-21-12-6-18(7-13-21)24(19-8-14-22(15-9-19)26(2)3)20-10-16-23(17-11-20)27(4)5;/h6-17H,1-5H3;1H

SMILES string

Cl.C\N=C1/C=C\C(C=C1)=C(/c2ccc(cc2)N(C)C)c3ccc(cc3)N(C)C

InChI key

JFTBTTPUYRGXDG-UHFFFAOYSA-N

agency

certified by the BSC

form

crystalline powder

composition

Dye content, 75%

technique(s)

microbe id | staining: suitable

mp

137 °C (dec.) (lit.)

λmax

584 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

Related Categories

General description

Methyl Violet 2B is a mixture of polymethylated (4,5 and 6) pararosaniline chlorides

Application

Certified for use in Flemming triple stain with iodine for chromosomes; Newton′s crystal violet-iodine technique for chromatin and nucleoli; Lieb′s amyloid stain.
Methyl violet 2B has been used to stain human lung fibroblast cells (A549) in in vitro antiviral assay.

Biochem/physiol Actions

Methyl violet 2B is a cationic dye with a triphenylmethane structure. It acts as a selective dye and facilitates the differentiation of Klebsiella pneumoniae from Enterobacter aerogenes and other gram-negative organisms. Methyl violet 2B is harmful if inhaled, swallowed or absorbed through skin, contact may cause damage to skin and eyes. Hence, the dye removal is of great importance.

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SHBR1833
MKBD5246V
MKBG5819V
MKBF7373V
MKBD5246

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Annabelle Herrington-Symes et al.
Journal of drug targeting, 25(9-10), 881-890 (2017-08-19)
Increasingly complex proteins can be made by a recombinant chemical approach where proteins that can be made easily can be combined by site-specific chemical conjugation to form multifunctional or more active protein therapeutics. Protein dimers may display increased avidity for
Equilibrium and kinetic studies of methyl violet sorption by agricultural waste
Hameed B H
Journal of Hazardous Materials, 154(1-3), 204-212 (2008)
Methyl Violet: a Selective Agent for Differentiation of
Klebsiella pneumoniae from Enterobacter aerogenes and Other
Gram-Negative Organisms
CAMPBELL L M and ROTH I L
Applied Microbiology, 30, 258-261 (1975)
Expression of soluble and active interferon
consensus in SUMO fusion expression system
in E.coli
Peciak K
Protein Expression and Purification, 99 (2014), 18- 26 (2014)
Removal of cationic dye methyl violet 2B from water by cation exchange membranes
ShiouWu J
Journal of Membrane Science, 309(1-2), 239-245 (2008)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service