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Sigma-Aldrich

Brilliant Black BN

Dye content 60 %

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Synonym(s):
Food Black 1
Empirical Formula (Hill Notation):
C28H17N5Na4O14S4
CAS Number:
Molecular Weight:
867.68
Colour Index Number:
28440
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.47

form

powder

Quality Level

composition

Dye content, 60%

λmax

407 nm (2nd)
570 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].CC(=O)Nc1ccc(c2cc(c(\N=N\c3ccc(\N=N/c4ccc(cc4)S([O-])(=O)=O)c5ccc(cc35)S([O-])(=O)=O)c(O)c12)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C28H21N5O14S4.4Na/c1-14(34)29-23-10-11-24(50(42,43)44)20-13-25(51(45,46)47)27(28(35)26(20)23)33-32-22-9-8-21(18-7-6-17(12-19(18)22)49(39,40)41)31-30-15-2-4-16(5-3-15)48(36,37)38;;;;/h2-13,35H,1H3,(H,29,34)(H,36,37,38)(H,39,40,41)(H,42,43,44)(H,45,46,47);;;;/q;4*+1/p-4/b31-30-,33-32+;;;;

InChI key

GMMAPXRGRVJYJY-OXYXOBGMSA-J

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Application

Brilliant Black BN has been used:
  • in micronucleus and comet assays to evaluate its genotoxic effect.
  • as a quenching agent in calcium mobilization assay.
  • as a redox indicator in microbiological inhibition bioassay using Bacillus megaterium to detect tetracycline and macrolides in milk.

Biochem/physiol Actions

[4-acetamido-5-hydroxy-6-[7-sulfonato-4-(4-sulfonatophenylazo)-1-naphtylazo], also known as food black 1, belongs to the group of azo dyes. Brilliant Black BN is widely used as a food dye and pharmaceutical excipient. In addition, it also acts as an allosteric modulator of the adenosine A1 and A3 receptors and is generally used within calcium mobilization assays to reduce extracellular fluorescence.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Antagonist selective modulation of adenosine A1 and A3 receptor pharmacology by the food dye Brilliant Black BN: evidence for allosteric interactions.
May LT
Molecular Pharmacology, 77, 678-686 (2010)
Novel bioassay using Bacillus megaterium to detect tetracycline in milk
Tumini M
Revista Argentina de microbiologia, 48, 143-146 (2016)
The evaluation of the genotoxicity of two commonly used food colors: Quinoline Yellow (E 104) and Brilliant Black BN (E 151).
Cellular & Molecular Biology Letters, 9, 107-122 (2004)
Calcium Mobilisation Assay in Response to Chemokine Stimulation
Cronshaw DG
Bio-protocol, 2 (2012)
Microbiological Method using Bacillus megaterium
with Fusidic Acid for Detection of Macrolides in Milk
Tumin M
Czech Journal of Food Sciences, 34, 9-15 (2016)

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