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211842

Brilliant Black BN

Dye content 60 %

Synonym(s):

Food Black 1

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About This Item

Empirical Formula (Hill Notation):
C28H17N5Na4O14S4
CAS Number:
2519-30-4
Molecular Weight:
867.68
PubChem Substance ID:
24852710
UNSPSC Code:
12171500
Colour Index Number:
28440
NACRES:
NA.47
EC Number:
219-746-5
MDL number:
MFCD00078980
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form

powder

Quality Level

200

composition

Dye content, 60%

λmax

407 nm (2nd), 570 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].CC(=O)Nc1ccc(c2cc(c(\N=N\c3ccc(\N=N/c4ccc(cc4)S([O-])(=O)=O)c5ccc(cc35)S([O-])(=O)=O)c(O)c12)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C28H21N5O14S4.4Na/c1-14(34)29-23-10-11-24(50(42,43)44)20-13-25(51(45,46)47)27(28(35)26(20)23)33-32-22-9-8-21(18-7-6-17(12-19(18)22)49(39,40)41)31-30-15-2-4-16(5-3-15)48(36,37)38;;;;/h2-13,35H,1H3,(H,29,34)(H,36,37,38)(H,39,40,41)(H,42,43,44)(H,45,46,47);;;;/q;4*+1/p-4/b31-30-,33-32+;;;;

InChI key

GMMAPXRGRVJYJY-OXYXOBGMSA-J

Application

Brilliant Black BN has been used:
  • in micronucleus and comet assays to evaluate its genotoxic effect.
  • as a quenching agent in calcium mobilization assay.
  • as a redox indicator in microbiological inhibition bioassay using Bacillus megaterium to detect tetracycline and macrolides in milk.

Biochem/physiol Actions

[4-acetamido-5-hydroxy-6-[7-sulfonato-4-(4-sulfonatophenylazo)-1-naphtylazo], also known as food black 1, belongs to the group of azo dyes. Brilliant Black BN is widely used as a food dye and pharmaceutical excipient. In addition, it also acts as an allosteric modulator of the adenosine A1 and A3 receptors and is generally used within calcium mobilization assays to reduce extracellular fluorescence.

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Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT2811
SHBQ6363
SHBN6839
SHBL4641
MKCC1198

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Antagonist selective modulation of adenosine A1 and A3 receptor pharmacology by the food dye Brilliant Black BN: evidence for allosteric interactions.
May LT
Molecular Pharmacology, 77, 678-686 (2010)
Microbiological Method using Bacillus megaterium
with Fusidic Acid for Detection of Macrolides in Milk
Tumin M
Czech Journal of Food Sciences, 34, 9-15 (2016)
Andrew Joiner et al.
Journal of dentistry, 36 Suppl 1, S8-14 (2008-07-23)
To investigate a new optical approach to tooth whitening by enhancing the measurement and perception of tooth whiteness using blue coloured materials deposited onto the tooth surface. Salivary pellicle coated human extracted teeth or polished enamel specimens were used as
View All Related Papers

Global Trade Item Number

SKUGTIN
211842-10G04061838771988