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Merck
CN

22150

D-(+)-Cellobiose

≥99.0%, suitable for microbiology, Reducing sugar disaccharide

Synonym(s):

Cellobiose, Cellose, Beta-Cellobiose, D-Cellobiose, β-D-Glc-(1→4)-D-Glc, 4-O-β-D-Glucopyranosyl-D-glucose

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About This Item

Empirical Formula (Hill Notation):
C12H22O11
CAS Number:
528-50-7
Molecular Weight:
342.30
UNSPSC Code:
41106212
NACRES:
NA.85
PubChem Substance ID:
329752272
EC Number:
208-436-5
Beilstein/REAXYS Number:
93795
MDL number:
MFCD00136034

Key Documents

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Product Name

D-(+)-Cellobiose, ≥99.0%, suitable for microbiology, Reducing sugar disaccharide

InChI key

DKXNBNKWCZZMJT-WELRSGGNSA-N

InChI

1S/C12H22O11/c13-1-4(16)7(18)11(5(17)2-14)23-12-10(21)9(20)8(19)6(3-15)22-12/h1,4-12,14-21H,2-3H2/t4-,5+,6+,7+,8+,9-,10+,11+,12-/m0/s1

SMILES string

OC[C@@H](O)[C@@H](O[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O)[C@H](O)[C@@H](O)C=O

assay

≥99.0% (HPLC)
≥99.0%

form

powder

optical activity

[α]20/D +34±1°, 15 hr, c = 10% in H2O

mol wt

342.30 g/mol

ign. residue

≤0.1% (as SO4)

loss

≤1% loss on drying, 110 °C

mp

~235 °C (dec.)
239 °C (dec.) (lit.)

anion traces

chloride (Cl-): ≤50 mg/kg
sulfate (SO42-): ≤50 mg/kg

cation traces

As: ≤0.1 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤150 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Zn: ≤5 mg/kg

application(s)

microbiology

Quality Level

200

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Application

Cellobiose has applications as a carbohydrate source in culture media and biochemical characterization of microorganisms, based on their carbohydrate fermentation abilities

General description

Cellobiose is a disaccharide that is a reducing sugar. It is added to culture media as a nutrient supplement, specifically as a carbohydrate source, and can be used to differentiate bacteria based on their ability to ferment cellobiose. For example, Vibrio spp. can ferment cellobiose which results in acid production and can be indicated by the pH indicator bromothymol blue, which turns yellow at acidic pH. The addition of cellobiose in the media also stimulates the initial growth of Salmonella.

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP0000
BCCN5922
BCCN1423
BCCL4941
BCCL7767

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Benjamin J Matthews et al.
eLife, 8 (2019-05-22)
Female Aedes aegypti mosquitoes are deadly vectors of arboviral pathogens and breed in containers of freshwater associated with human habitation. Because high salinity is lethal to offspring, correctly evaluating water purity is a crucial parenting decision. We found that the
Corinna Schiano-di-Cola et al.
The FEBS journal, 287(12), 2577-2596 (2019-11-23)
Thermostable cellulases from glycoside hydrolase family 7 (GH7) are the main components of enzymatic mixtures for industrial saccharification of lignocellulose. Activity improvement of these enzymes via rational design is a promising strategy to alleviate the industrial costs, but it requires
Sylvie Estrela et al.
eLife, 10 (2021-04-21)
A major open question in microbial community ecology is whether we can predict how the components of a diet collectively determine the taxonomic composition of microbial communities. Motivated by this challenge, we investigate whether communities assembled in pairs of nutrients
Alessandro Boianelli et al.
PloS one, 7(10), e47393-e47393 (2012-10-31)
When grown on glucose and beta-glucosides, S. pneumoniae shows sequential use of sugars resulting in diauxic growth with variable time extent of the lag phase separating the biphasic growth curve. The pneumococcal beta-glucoside uptake locus containing the PTS transporter spr0276-82
Lara Hassan et al.
mBio, 10(4) (2019-07-04)
It is essential for microbes to acquire information about their environment. Fungi use soluble degradation products of plant cell wall components to understand the substrate composition they grow on. Individual perception pathways have been well described. However, the interconnections between
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Articles

Base Ingredients of Microbiology Media

Culture media provides a habitat with suitable nutrients, energy sources, and certain environmental conditions for the growth of microorganisms. The components of the culture media range from simple sugars to peptones, salts, antibiotics, and complex indicators.

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