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Merck
CN

222461

Murexide

ACS reagent

Synonym(s):

5,5′-Nitrilodibarbituric acid monoammonium salt, Ammonium purpurate

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About This Item

Empirical Formula (Hill Notation):
C8H8N6O6
CAS Number:
3051-09-0
Molecular Weight:
284.19
EC Number:
221-266-6
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24853333
MDL number:
MFCD00012777
Colour Index Number:
56085
Beilstein/REAXYS Number:
3831445

Key Documents

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Product Name

Murexide, ACS reagent

form

powder

InChI key

LJYRLGOJYKPILZ-UHFFFAOYSA-N

InChI

1S/C8H5N5O6.H3N/c14-3-1(4(15)11-7(18)10-3)9-2-5(16)12-8(19)13-6(2)17;/h(H2,10,11,14,15,18)(H3,12,13,16,17,19);1H3

SMILES string

N.OC1=C(\N=C2\C(=O)NC(=O)NC2=O)C(=O)NC(=O)N1

grade

ACS reagent

technique(s)

titration: suitable

mp

>300 °C (lit.)

λmax

525 nm

suitability

passes test for complexometric indicator

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Application

Murexide is used for the histochemical staining of calcium in the skeleton. It is suitable for staining the hemijaws for dental caries scoring.

General description

Murexide is the ammonium salt of purpuric acid that appears as a slightly reddish-purple powder and is soluble in water. It is mainly used as an indicator for complexometric titrations to measure water hardness and as a colorant/pigment in the dye industry.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN4176
BCCM1568
BCCL1516
BCCH6887
BCCF9241

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R De Giovanni et al.
Physiological chemistry and physics and medical NMR, 24(4), 271-280 (1992-01-01)
In this paper the authors studied the effects of thyroid hormones and their structural analogues on the mitochondrial calcium transport activities. The thyroid hormones, 3,5,3' L-triiodothyronine (LT3) and 3,5,3'5' L-tetraiodothyronine (LT4) at physiological intracellular concentrations between 7.2 and 9 nM
Cédric R H Lamboley et al.
Cell calcium, 50(6), 530-547 (2011-11-01)
One aim of this article was to determine the resting concentration of free Ca(2+) in the sarcoplasmic reticulum (SR) of frog cut skeletal muscle fibers ([Ca(2+)](SR,R)) using the calcium absorbance indicator dye tetramethylmurexide (TMX). Another was to determine the ratio
Shao-Kai Sun et al.
The Analyst, 137(9), 2124-2128 (2012-03-23)
A simple and efficient colorimetric method for the naked-eye detection and quantification of histidine in biological fluids was developed based on an indicator-displacement assay (IDA) and the Ni(2+)-histidine affinity pair. In this IDA approach, a commercially available dye, murexide, was
J Maylie et al.
The Journal of general physiology, 89(1), 145-176 (1987-01-01)
The Ca indicator tetramethylmurexide was introduced into cut fibers, mounted in a double-Vaseline-gap chamber, by diffusion from the end-pool solutions. The indicator diffused rapidly to the central region of a fiber where optical recording was done and, if removed, diffused
A Hirota et al.
The Journal of general physiology, 94(4), 597-631 (1989-10-01)
Two new Ca indicators, purpurate-3,3'diacetic acid (PDAA) and 1,1'-dimethylpurpurate-3,3'diacetic acid (DMPDAA), were synthesized and used to measure Ca transients in frog cut muscle fibers. These indicators are analogues of the purpurate components of murexide and tetramethylmurexide, in which two acetate
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