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Merck
CN

248266

Xylidyl blue I

synthetic colorimetric reagent

Synonym(s):

4-Hydroxy-3-[2-hydroxy-3-(2,4-dimethylphenylaminocarbonyl)-1-naphthylazobenzenesulfonic acid sodium salt, Magonsulfonate

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About This Item

Linear Formula:
HOC6H3[N=NC10H5(OH)[CONHC6H3(CH3)2]]SO3Na
CAS Number:
Molecular Weight:
513.50
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
239-012-8
Beilstein/REAXYS Number:
4107363
MDL number:
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Quality Level

form

powder or crystals

technique(s)

titration: suitable

color

dark violet

λmax

612 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].Cc1ccc(NC(=O)c2cc3ccccc3c(\N=N\c4cc(ccc4O)S([O-])(=O)=O)c2O)c(C)c1

InChI

1S/C25H21N3O6S.Na/c1-14-7-9-20(15(2)11-14)26-25(31)19-12-16-5-3-4-6-18(16)23(24(19)30)28-27-21-13-17(35(32,33)34)8-10-22(21)29;/h3-13,29-30H,1-2H3,(H,26,31)(H,32,33,34);/q;+1/p-1/b28-27+;

InChI key

LKSHJHJGLORTGD-RXQWRGDBSA-M

General description

Xylydil Blue I is a synthetic colorimetric reagent that has been widely used for the spectrophotometric detection of magnesium.

Application

Xylydil Blue I is suitable to determine serum magnesium levels using Mg- Xylydil Blue I complex based method.

Biochem/physiol Actions

Xylydil Blue I forms a purple complex with the magnesium ion in alkaline conditions.


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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[Measurement of magnesium in the human sera with xylidyl-blue I. I. The effect of phosphate ion].
Y Takubo et al.
Rinsho byori. The Japanese journal of clinical pathology, 31(11), 1244-1248 (1983-11-01)
Measurement of magnesium in serum and urine with a random access analyzer by use of a modified xylidyl blue-1 procedure.
D Ratge et al.
Clinica chimica acta; international journal of clinical chemistry, 159(2), 197-203 (1986-09-15)
M J Stone et al.
Clinical chemistry, 42(9), 1474-1477 (1996-09-01)
We have evaluated an enzymatic assay for the determination of Mg in serum, based on the activation of a chemically modified form of isocitrate dehydrogenase by Mg2+. In the presence of potassium isocitrate and NADP+, NADPH, which absorbs light at



Global Trade Item Number

SKUGTIN
248266-5G04061825927817
248266-1G04061826111970