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Chlorophosphonazo III

for spectrophotometric det. of alkaline earth metals

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Synonym(s):
Bis(4-chloro-2-phosphonobenzolazo)chromotropic acid
Empirical Formula (Hill Notation):
C22H16Cl2N4O14P2S2
CAS Number:
1914-99-4
Molecular Weight:
757.36
Beilstein:
2934871
EC Number:
217-628-8
MDL number:
MFCD00042698
PubChem Substance ID:
329752849
NACRES:
NA.21

form

powder

Quality Level

100

quality

for spectrophotometric det. of alkaline earth metals

technique(s)

UV/Vis spectroscopy: suitable

SMILES string

Oc1c(\N=N\c2ccc(Cl)cc2P(O)(O)=O)c(cc3cc(c(\N=N\c4ccc(Cl)cc4P(O)(O)=O)c(O)c13)S(O)(=O)=O)S(O)(=O)=O

InChI

1S/C22H16Cl2N4O14P2S2/c23-10-1-3-12(14(7-10)43(31,32)33)25-27-19-16(45(37,38)39)5-9-6-17(46(40,41)42)20(22(30)18(9)21(19)29)28-26-13-4-2-11(24)8-15(13)44(34,35)36/h1-8,29-30H,(H2,31,32,33)(H2,34,35,36)(H,37,38,39)(H,40,41,42)/b27-25+,28-26+

InChI key

LMBABJNSZGKTBA-NBHCHVEOSA-N

Related Categories

General description

Chlorophosphonazo III is a highly sensitive reagent and can be used for the spectrophotometric determination of zirconium, titanium, thorium and scandium. It also finds application as an extractant, along with 1-butanol as solvent, for determination of micromolar concentrations of Nd(III), Th(IV) and U(VI) in brine. Above all that, it has two long wavelength maxima, one at 620 nm and one at 680 nm.

Application

Chlorophosphonazo III was used as a chromogenic agent along with PVC, during the determination of calcium via a disposable optical sensor.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Reagent for the spectrophotometric determination of alkaline earth metals

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Rapid changes in intracellular free calcium concentration. Detection by metallochromic indicator dyes in squid giant axon.
Brown JE
Biophysical Journal, 15 (11), 1155-1155 (1975)
Determination of calcium by a single-use optical sensor.
Capitan VLF
Sensors and Actuators B, Chemical, 71 (1), 140-146 (2000)
M. Zenki
Analytical Chemistry, 53, 968-968 (1981)
S M Baylor et al.
The Journal of physiology, 331, 179-210 (1982-10-01)
1. Absorbance changes were measured following stimulation of single muscle fibres injected with the metallochromic indicator dye Arsenazo III. Two dye-related signals can be clearly resolved: (1) an early, transient isotropic signal that appears to be due to the formation
Wei-Wen Liu et al.
Biomedical optics express, 12(2), 1154-1166 (2021-03-09)
This study demonstrates that chlorophosphonazo III (CPZ III) can be used as a contrast agent for photoacoustic calcium imaging. CPZ III can pass across the plasma membrane for labeling intracellular Ca2+ without cytotoxicity. In optical-resolution photoacoustic microscopy (OR-PAM), the photoacoustic
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