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Sigma-Aldrich

Chromotrope 2B

for microscopy

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Synonym(s):
2-(4-Nitrophenylazo)chromotropic acid disodium salt, Acid Red 176
Empirical Formula (Hill Notation):
C16H9N3Na2O10S2
CAS Number:
548-80-1
Molecular Weight:
513.37
Colour Index Number:
16575
Beilstein:
4109733
EC Number:
208-959-9
MDL number:
MFCD00003951
PubChem Substance ID:
329752979
NACRES:
MA.02

grade

for microscopy

Quality Level

100

form

powder or crystals

mp

300 °C

solubility

water: 10 mg/mL

ε (extinction coefficient)

≥500 at 509-519 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].[Na+].Oc1cc(cc2cc(c(\N=N\c3ccc(cc3)[N+]([O-])=O)c(O)c12)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C16H11N3O10S2.2Na/c20-12-7-11(30(24,25)26)5-8-6-13(31(27,28)29)15(16(21)14(8)12)18-17-9-1-3-10(4-2-9)19(22)23;;/h1-7,20-21H,(H,24,25,26)(H,27,28,29);;/q;2*+1/p-2/b18-17+;;

InChI key

AXUUHWJXDWBCSG-QIKYXUGXSA-L

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Application

Chromotrope 2B along with Chromotrope 2R, was used to give a highly colored radical anion, during the spectrophotometric determination of cephalosporins (cephadroxil (I), cephalexin (II) and cephradine (III)) in pharmaceutical products. It may be immobilized on AG 1-X8 ion exchange resin, used in flame atomic absorption spectrometry and inductively coupled plasma mass spectrometry (ICP-MS), done for the on-line preconcentration and determination of aluminium.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Spectrophotometric assay of cephalosporins in pharmaceutical products, using chromotrope 2B and chromotrope 2R.
Issa YM and Amin AS
Microchimica Acta, 124 (3-4), 203-209 (1996)
On-line preconcentration of aluminium with immobilized Chromotrope 2B for the determination by flame atomic absorption spectrometry and inductively coupled plasma mass spectrometry.
Martin-esteban A
Analytica Chimica Acta, 304 (1), 121-126 (1995)

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