318337
Acridine Orange hydrochloride hydrate
≥98% (HPLC)
Synonym(s):
3,6-Bis(dimethylamino)acridine hydrochloride
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About This Item
Empirical Formula (Hill Notation):
C17H19N3 · HCl · xH2O
CAS Number:
Molecular Weight:
301.81 (anhydrous basis)
EC Number:
200-614-0
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
MDL number:
Colour Index Number:
46005
Beilstein/REAXYS Number:
3734978
Quality Level
assay
≥98% (HPLC)
form
powder
technique(s)
microbe id | staining: suitable
color
orange
mp
284-287 °C (lit.)
solubility
H2O: 0.1%, clear, orange to red
λmax
492 nm
ε (extinction coefficient)
≥14,000 at 227-233 nm in water at 0.005 g/L, ≥35000 at 265-271 nm in water at 0.005 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Cl[H].[H]O[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C
InChI
1S/C17H19N3.ClH.H2O/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;/h5-11H,1-4H3;1H;1H2
InChI key
JUAAUEXLEKAEQC-UHFFFAOYSA-N
Application
Acridine Orange hydrochloride hydrate dye has been used:
- to stain intracellular acidic vesicles.
- to measure lysosomal membrane permeability (LMP).
- or autophagy detection.
A rigorously purified zinc-free material suitable for use in flow cytometry.
Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.
Biochem/physiol Actions
Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.
signalword
Warning
hcodes
Hazard Classifications
Muta. 2
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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International Journal of Oncology, 45, 2331-2340 (2014)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 318337-1G | 04061826693353 |
| 318337-250MG | 04061833257920 |
| 318337-5G | 04061826693391 |