Merck
CN
All Photos(4)

Documents

318337

Sigma-Aldrich

Acridine Orange hydrochloride hydrate

≥98% (HPLC)

Sign Into View Organizational & Contract Pricing
All Photos(4)
Synonym(s):
3,6-Bis(dimethylamino)acridine hydrochloride
Empirical Formula (Hill Notation):
C17H19N3 · HCl · xH2O
CAS Number:
65-61-2
Molecular Weight:
301.81 (anhydrous basis)
Colour Index Number:
46005
Beilstein:
3734978
EC Number:
200-614-0
MDL number:
MFCD00150070
PubChem Substance ID:
24859123
NACRES:
NA.47

Quality Level

200

Assay

≥98% (HPLC)

form

powder

color

orange

mp

284-287 °C (lit.)

solubility

H2O: 0.1%, clear, orange to red

λmax

492 nm

ε (extinction coefficient)

≥14,000 at 227-233 nm in water at 0.005 g/L
≥35000 at 265-271 nm in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl[H].[H]O[H].CN(C)c1ccc2cc3ccc(cc3nc2c1)N(C)C

InChI

1S/C17H19N3.ClH.H2O/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;;/h5-11H,1-4H3;1H;1H2

InChI key

JUAAUEXLEKAEQC-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Application

A rigorously purified zinc-free material suitable for use in flow cytometry.
Acridine Orange hydrochloride hydrate dye has been used:
  • to stain intracellular acidic vesicles.
  • to measure lysosomal membrane permeability (LMP).
  • or autophagy detection.
Fluorescent stain for nucleic acids in agarose and polyacrylamide gels. Has been used to study apoptosis in various cell culture and tissue models.

Biochem/physiol Actions

Acridine Orange is a metachromatic dye which can stain DNA, RNA and acid glycosaminoglycans. At low concentrations, it intercalates into DNA and precipitates RNA. However, at high concentrations it denatures and precipitates both RNA and DNA. Acridine Orange is also used to analyze autophagy. It enters into acidic organelles in a pH-dependent manner. At neutral pH, acridine orange gives a green fluorescence and in acidic conditions, it accumulates in acidic organelles giving a bright red fluorescence.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H341

Hazard Classifications

Muta. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Michael M Fisher et al.
International journal of radiation oncology, biology, physics, 107(4), 793-803 (2020-04-17)
We examined the capacity of the pan-fibroblast growth factor receptor (FGFR) inhibitor AZD4547 to augment radiation response across a panel of head and neck squamous cell carcinoma (HNSCC) cell lines and xenografts. FGFR1, FGFR2, and FGFR3 RNA in situ hybridization
Autophagy-dependent senescence in response to DNA damage and chronic apoptotic stress
Singh K, et.al.
Autophagy, 8, 236-251 (2012)
Autophagy inhibition uncovers the neurotoxic action of the antipsychotic drug olanzapine
Vucicevic L
Autophagy, 10, 2362-2378 (2014)
F. W. D. Rost
Fluorescence Microscopy, 2 (1992)
Viriditoxin regulates apoptosis and autophagy via mitotic catastrophe and microtubule formation in human prostate cancer cells.
Kundu S, et.al.
International Journal of Oncology, 45, 2331-2340 (2014)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service