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318752

Sigma-Aldrich

Bromothymol Blue sodium salt solution

0.04 wt. % in H2O

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Empirical Formula (Hill Notation):
C27H27Br2NaO5S
CAS Number:
34722-90-2
Molecular Weight:
646.36
MDL number:
MFCD00077263
PubChem Substance ID:
24859144
NACRES:
NA.47

form

liquid

Quality Level

200

concentration

0.04 wt. % in H2O

pH

6.0-7.6, yellow to blue

density

0.99 g/mL at 25 °C (lit.)

λmax

615 nm

ε (extinction coefficient)

≥13000 at 304-310 nm in 0.1 M NaOH at 0.005 g/L
≥8000 at 391-397 nm in 0.1 M NaOH at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CC(C)c1cc(c(C)c(Br)c1O)\C(=C2/C=C(C(C)C)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O

InChI

1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;

InChI key

NMKFVGALBGZKGW-FKWCIMQXSA-M

Related Categories

General description

Bromothymol Blue is a sulfonated hydroxyquinone in acidic solutions. In alkaline solutions, it is present as a dianion that is blue with a larger conjugated system than the yellow hydroxyquinone. Both forms are lipophilic. It is also known as bromthymol blue or dibromothymolsulfonphthalein.

Application

Bromothymol blue has various applications in biology and medicine. It is suitable for demonstrating fungal hyphae within plant roots. It is used as a vital stain to trace the movement of fluids from the lymph. It is added to an agar gel microbial culture medium for enumeration of Bacillus cereus. Bromothymol blue is also used as a pH indicator due to its precise color transition properties at neutrality.

Biochem/physiol Actions

Bromothymol Blue is a pH indicator which changes color from yellow (pH 6.0) to blue (7.6). It belongs to the class of sulfonephthalein dyes.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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