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Merck
CN

32750

3,3′-Diaminobenzidine tetrahydrochloride hydrate

for spectrophotometric det. of Se, ≥97.5%

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About This Item

Linear Formula:
C12H14N4 · 4HCl · xH2O
CAS Number:
868272-85-9
NACRES:
NA.21
PubChem Substance ID:
329754314
UNSPSC Code:
41116105
EC Number:
231-018-9
MDL number:
MFCD08273058

Key Documents

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InChI key

DXWSCXIZIHILNP-UHFFFAOYSA-N

InChI

1S/C12H14N4.4ClH.H2O/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;;/h1-6H,13-16H2;4*1H;1H2

SMILES string

O.Cl.Cl.Cl.Cl.Nc1ccc(cc1N)-c2ccc(N)c(N)c2

assay

≥97.5%

quality

for spectrophotometric det. of Se

technique(s)

UV/Vis spectroscopy: suitable

Quality Level

100

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General description

3,3′-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) is toxic in nature, and is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels.

Application

3,3′-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex.

Other Notes

Reagent for the spectrophotometric determination of selenium; Peroxidase stain. Differentiation between two classes of mycobacterial catalase in polyacrylamide electrophoresis gels; Specific indication of hemoproteins in polyacrylamide gels by double staining

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

危险化学品
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Certificates of Analysis (COA)

Lot/Batch Number
MKCZ1210
MKCX2929
MKCV5872
MKCW7760
MKCK5098

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HistoGreen: a new alternative to 3, 3'-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) as a peroxidase substrate in immunohistochemistry"
Thomas MA and Lemmer B
Brain Research, 14 (2), 107-118 (2005)
R T Francis et al.
Analytical biochemistry, 136(2), 509-514 (1984-02-01)
Hemoproteins were revealed in polyacrylamide gels in the presence of sodium dodecyl sulfate by staining with different benzidine derivatives. When the protein samples were treated with either beta-mercaptoethanol or dithiothreitol, a significant decrease in peroxidase activity of the proteins possessing
Interleukin-8 in the Human Endometrium 1.
Ariciri A
J. Clin. Endocr., 83 (5), 1783-1787 (1998)
Danzuka, T. and Ueno, K.
Analytical Chemistry, 30, 1370-1370 (1958)
L G Wayne et al.
Analytical biochemistry, 157(1), 89-92 (1986-08-15)
Mycobacteria produce two classes of catalase, designated T and M. Only the T-catalase also has a peroxidase-like function. When a 3,3'-diaminobenzidine (DAB) peroxidase stain was applied to polyacrylamide gel electrophoresis gels, followed by a ferricyanide negative stain for catalase, isoenzymes
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