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Merck
CN

33152

1,5-Diphenylcarbazide

reag. Ph. Eur., ≥98.0%

Synonym(s):

1,5-Diphenylcarbohydrazide, sym.-Diphenylcarbazide

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About This Item

Linear Formula:
C6H5NHNHCONHNHC6H5
CAS Number:
140-22-7
Molecular Weight:
242.28
UNSPSC Code:
41116105
NACRES:
NA.21
PubChem Substance ID:
329754464
EC Number:
205-403-7
Beilstein/REAXYS Number:
752039
MDL number:
MFCD00003013
Assay:
≥98% (HPLC), ≥98.0%

Key Documents

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InChI key

KSPIHGBHKVISFI-UHFFFAOYSA-N

InChI

1S/C13H14N4O/c18-13(16-14-11-7-3-1-4-8-11)17-15-12-9-5-2-6-10-12/h1-10,14-15H,(H2,16,17,18)

SMILES string

O=C(NNc1ccccc1)NNc2ccccc2

agency

USP/NF, reag. Ph. Eur.

assay

≥98% (HPLC), ≥98.0%

technique(s)

UV/Vis spectroscopy: suitable, titration: suitable

impurities

diphenylcarbazone, in accordance, ≤0.1% insoluble matter in ethanol

ign. residue

≤0.05% (as SO4)

Quality Level

200

suitability

passes test for Hg detection

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General description

1,5-Diphenylcarbazide reaction with chromium(VI), has been widely used for the spectro- photometric determination of chromium. It is also an electron donor, and can be used for measuring photosystem 2 activity in subchloroplast fragments.

Application

1,5-diphenylcarbazide was used for preparing diphenylcarbazide solution, during an experimental procedure done for reducing biological chromium(VI), using trickling filter. It may be used for pre-column complexation in HPLC, during on-line preconcentration and determination of chromium(VI) traces in water.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL6021
BCCH0705
BCCD4110
BCCC2184
BCBV5957

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Biological chromium (VI) reduction using a trickling filter.
Dermou E
Journal of Hazardous Materials, 126 (1), 78-85 (2005)
On-line preconcentration and determination of chromium (VI) in waters by high-performance liquid chromatography using pre-column complexation with 1, 5-diphenylcarbazide.
Padarauskas A
Journal of Chromatography A, 808 (1), 193-199 (1998)
Oxidation of 1, 5-diphenylcarbazide as a measure of photosystem 2 activity in subchloroplast fragments.
Vernon LP and Shaw ER.
Biochemical and Biophysical Research Communications, 36 (6), 878-884 (1969)
N Rajesh et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 69(2), 612-618 (2007-07-03)
A novel approach has been developed for the solid phase extraction of chromium(VI) based on the adsorption of its diphenylcarbazide complex on a mixture of acid activated montmorillonite (AAM)-silica gel column. The effect of various parameters such as acidity, stability
A Padarauskas et al.
Journal of chromatography. A, 808(1-2), 193-199 (1998-07-04)
A method for on-line chromatographic preconcentration and determination of chromium(VI) traces has been developed. Chromate was preconcentrated on a C18 column (50 x 6 mm I.D.) after complexation with diphenylcarbazide (DPC). Following the preconcentration step, analysis of the sample was
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