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Merck
CN

33152

1,5-Diphenylcarbazide

reag. Ph. Eur., ≥98.0%

Synonym(s):

1,5-Diphenylcarbohydrazide, sym.-Diphenylcarbazide

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About This Item

Linear Formula:
C6H5NHNHCONHNHC6H5
CAS Number:
140-22-7
Molecular Weight:
242.28
UNSPSC Code:
41116105
NACRES:
NA.21
PubChem Substance ID:
329754464
EC Number:
205-403-7
Beilstein/REAXYS Number:
752039
MDL number:
MFCD00003013
Assay:
≥98% (HPLC), ≥98.0%

Key Documents

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InChI key

KSPIHGBHKVISFI-UHFFFAOYSA-N

InChI

1S/C13H14N4O/c18-13(16-14-11-7-3-1-4-8-11)17-15-12-9-5-2-6-10-12/h1-10,14-15H,(H2,16,17,18)

SMILES string

O=C(NNc1ccccc1)NNc2ccccc2

agency

USP/NF, reag. Ph. Eur.

assay

≥98% (HPLC), ≥98.0%

technique(s)

UV/Vis spectroscopy: suitable, titration: suitable

impurities

diphenylcarbazone, in accordance, ≤0.1% insoluble matter in ethanol

ign. residue

≤0.05% (as SO4)

Quality Level

200

suitability

passes test for Hg detection

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General description

1,5-Diphenylcarbazide reaction with chromium(VI), has been widely used for the spectro- photometric determination of chromium. It is also an electron donor, and can be used for measuring photosystem 2 activity in subchloroplast fragments.

Application

1,5-diphenylcarbazide was used for preparing diphenylcarbazide solution, during an experimental procedure done for reducing biological chromium(VI), using trickling filter. It may be used for pre-column complexation in HPLC, during on-line preconcentration and determination of chromium(VI) traces in water.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL6021
BCCH0705
BCCD4110
BCCC2184
BCBV5957

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Biological chromium (VI) reduction using a trickling filter.
Dermou E
Journal of Hazardous Materials, 126 (1), 78-85 (2005)
On-line preconcentration and determination of chromium (VI) in waters by high-performance liquid chromatography using pre-column complexation with 1, 5-diphenylcarbazide.
Padarauskas A
Journal of Chromatography A, 808 (1), 193-199 (1998)
Oxidation of 1, 5-diphenylcarbazide as a measure of photosystem 2 activity in subchloroplast fragments.
Vernon LP and Shaw ER.
Biochemical and Biophysical Research Communications, 36 (6), 878-884 (1969)
W Hillier et al.
Biochemistry, 36(1), 76-85 (1997-01-07)
The behavior of photosystem II (PSII) reactions was investigated under conditions of decreasing water content by the addition of increasing concentrations of ethylene glycol (EG). The photosynthetic activities were measured for PSII samples either directly in aqueous solutions of EG
G Samanta et al.
Analytical chemistry, 73(9), 2034-2040 (2001-05-17)
An automated continuous instrument for the collection and measurement of aerosol Cr(VI) is described. The system alternately collects the sample on one of two glass fiber filters. After 15-min sample collection on one filter, the sampling switches over to the
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