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Merck
CN

39955

Schaeffer and Fulton Spore Stain Solution B

Synonym(s):

Safranin O Solution according to Schaeffer and Fulton

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About This Item

CAS Number:
UNSPSC Code:
12171500
NACRES:
MA.02
MDL number:
Technical Service
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product line

BioChemika

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

suitability

suitable for microscopy, spore forming microorganisms

SMILES string

[Cl-].[n+]2(c3c(nc4c2cc(c(c4)C)N)c(c(cc3)N)C)c1ccccc1

InChI

1S/C20H18N4.ClH/c1-12-10-17-19(11-16(12)22)24(14-6-4-3-5-7-14)18-9-8-15(21)13(2)20(18)23-17;/h3-11,22H,21H2,1-2H3;1H

InChI key

QRYAEWIQIBAZOJ-UHFFFAOYSA-N

General description

In Schaeffer-Fulton`s method, a primary stain-malachite green is forced into the spore by steaming the bacterial emulsion. Malachite green (Schaeffer & Fulton`s Spore Stain A: Cat. No. 90903) is water soluble and has a low affinity for cellular material, so vegetative cells may be decolorized with water. Vegetative cells are then counterstained with safarin. The components and the method are also available as kit (Cat. No. 04551).

Other Notes

Schaeffer & Fulton`s Spore Stain B: SafarinO 5 g/l in water


pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves



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I L Erokhina et al.
Tsitologiia, 53(1), 39-43 (2011-04-09)
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Acta biomaterialia, 7(4), 1644-1652 (2010-12-07)
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Lauren N M Hayward et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 31(4), 567-573 (2012-11-21)
Assessment of the early stages of fracture healing via X-rays and computed tomography is limited by the low radio-opacity of cartilage. We validated a method of contrast-enhanced computed tomography (CECT) for non-destructive identification of cartilage within a healing fracture callus.



Global Trade Item Number

SKUGTIN
39955-100ML-F04061837824869