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Merck
CN

39955

Sigma-Aldrich

Schaeffer and Fulton Spore Stain Solution B

Synonym(s):

Safranin O Solution according to Schaeffer and Fulton

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About This Item

CAS Number:
477-73-6
MDL number:
MFCD06412419
UNSPSC Code:
12171500
NACRES:
MA.02

Key Documents

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product line

BioChemika

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

suitability

suitable for microscopy
spore forming microorganisms

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].[n+]2(c3c(nc4c2cc(c(c4)C)N)c(c(cc3)N)C)c1ccccc1

InChI

1S/C20H18N4.ClH/c1-12-10-17-19(11-16(12)22)24(14-6-4-3-5-7-14)18-9-8-15(21)13(2)20(18)23-17;/h3-11,22H,21H2,1-2H3;1H

InChI key

QRYAEWIQIBAZOJ-UHFFFAOYSA-N

General description

In Schaeffer-Fulton`s method, a primary stain-malachite green is forced into the spore by steaming the bacterial emulsion. Malachite green (Schaeffer & Fulton`s Spore Stain A: Cat. No. 90903) is water soluble and has a low affinity for cellular material, so vegetative cells may be decolorized with water. Vegetative cells are then counterstained with safarin. The components and the method are also available as kit (Cat. No. 04551).

Other Notes

Schaeffer & Fulton`s Spore Stain B: SafarinO 5 g/l in water

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN6646
BCCM1246
BCCN2014
BCCK8459
BCCJ2709

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