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Merck
CN

410217

2′,7′-Dichlorofluorescein

ACS reagent

Synonym(s):

Dichlorofluorescein; Fluorescein 27

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About This Item

Empirical Formula (Hill Notation):
C20H10Cl2O5
CAS Number:
Molecular Weight:
401.20
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
200-968-6
Beilstein/REAXYS Number:
58009
MDL number:
Technical Service
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grade

ACS reagent

Quality Segment

form

powder

technique(s)

titration: suitable

color

orange to red-brown, powder

mp

280 °C (dec.) (lit.)

solubility

alcohol: passes test

density

0.790 g/cm3

λmax

509 nm

suitability

passes test for adsorption indicator

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl

InChI

1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H

InChI key

VFNKZQNIXUFLBC-UHFFFAOYSA-N

General description

2′,7′-Dichlorofluorescein is an oxidation-sensitive fluorescent probe.

Application

2′,7′-Dichlorofluorescein has been used to measure ROS (reactive oxygen species) formation in cells. It has been used as a fluorogenic substrate to study the peroxidase activity of saliva samples and extracts obtained from submandibular glands.


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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves



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