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Merck
CN

415049

Aniline Blue diammonium salt

certified by the BSC

Synonym(s):

Ink Blue R; 4-[4-[[4-(4-aminooxysulfonylanilino)phenyl]-[4-(4-aminooxysulfonylphenyl)iminocyclohexa-2,5-dien-1-ylidene]methyl]anilino]benzenesulfonic acid

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About This Item

Empirical Formula (Hill Notation):
C37H32N5O9S3
CAS Number:
Molecular Weight:
786.87
MDL number:
UNSPSC Code:
12171500
NACRES:
NA.47
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SMILES string

[S](=O)(=O)(ON)c1ccc(cc1)Nc2ccc(cc2)C(=C5C=CC(=Nc6ccc(cc6)[S](=O)(=O)ON)C=C5)c3ccc(cc3)Nc4ccc(cc4)[S](=O)(=O)O

InChI

1S/C37H31N5O9S3/c38-50-53(46,47)35-21-15-32(16-22-35)41-29-9-3-26(4-10-29)37(25-1-7-28(8-2-25)40-31-13-19-34(20-14-31)52(43,44)45)27-5-11-30(12-6-27)42-33-17-23-36(24-18-33)54(48,49)51-39/h1-24,40-41H,38-39H2,(H,43,44,45)

InChI key

CSYADFXDZYPANK-UHFFFAOYSA-N

agency

certified by the BSC

form

powder, crystals or granules

composition

Dye content, 65%

color

brown to very dark brown, and Red to Very Dark Red and Red-Brown and Very Dark Red-Brown

ε (extinction coefficient)

15000 at 595-605 nm in water at 0.01 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

General description

Aniline Blue is the traditional name applied to anionic dyes produced by successive phenylation and sulfonation of basic fuchsine. It is a mixture of methyl blue (Cl 42780, Acid blue 93) and water blue (Cl 42755, Acid blue 22).

Application

Aniline blue (nominally as methyl blue or water blue) is a component of commonly used polychrome stains. Heidenhain′s AZAN stain and the Mallory′s polychrome stain both entail staining steps using aniline blue-orange G mixtures. Aniline blue is used in Gomori′s one-step trichrome stain and Mallory′s connective tissue stain for tissue including kidney and intestine.
Certified for use in Mallory′s connective tissue stain and Gomori′s one-step trichrome.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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The ACT domain (aspartate kinase, chorismate mutase and TyrA), an allosteric effector binding domain, is commonly found in amino acid metabolic enzymes. In addition to ACT domain-containing enzymes, plants have a novel family of ACT domain repeat (ACR) proteins, which

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