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Merck
CN

41782

MRS Agar

NutriSelect® Plus, from non-animal source(vegitone), powder, selective and differential for Lactobacillus spp., pack of 500 g, suitable for microbiology

Synonym(s):

DeMan, Rogosa and Sharpe Agar, Vegitone, Vegitone MRS Agar

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171605
Manufacturer/tradename:
NutriSelect® Plus
Form:
powder
Suitability:
selective and differential for Lactobacillus spp., selective by low pH for Bifidobacterium spp.
Application(s):
agriculture
bioburden testing
environmental
food and beverages, microbiology

Key Documents

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Quality Level

200

biological source

non-animal source (vegitone)

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L , ammonium citrate, 2.0 g/L , bromocresol green, 0.04 g/L , captan, 0.004 g/L , dextrose, 20.0 g/L , dipotassium phosphate, 2.0 g/L , magnesium sulfate, 0.1 g/L , manganese sulfate, 0.05 g/L , 2-phenylethyl alcohol, 3.0 g/L , proteose peptone (vegetable), 10.0 g/L , sodium acetate, 5.0 g/L , yeast extract, 5.0 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.5±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages, microbiology

suitability

selective and differential for Lactobacillus spp., selective by low pH for Bifidobacterium spp.

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General description

This MRS Agar is free of animal derived material. It is recommended for the isolation and cultivation of Lactobacillus species.

Application

MRS Agar, Vegitone has been used for culturing yogurt to analyze lactic acid bacteria (LAB). It has also been used in culturing of Lactobacillus reuteri colonies.

Preparation Note

Suspend 62.2 g in 1000 ml distilled water containing 1 ml polysorbate 80 (TWEEN® 80; Cat. No. P8074). Boil to dissolve the medium completely and mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN4982
BCCM7646
BCCM0516
BCCK3227
BCCJ9046

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He Ni et al.
Heliyon, 4(10), e00875-e00875 (2018-11-06)
In this study aqueous extracts from salal berry (SB) and blackcurrant pomace (BCP) were used to reformulate yogurt and the anti-diabetic properties of the beverage were investigated during 4 weeks of cold storage at 4 °C. Results indicated that α-amylase
Ferdinand Sedlmayer et al.
Nature communications, 9(1), 1822-1822 (2018-05-10)
Quorum sensing is a promising target for next-generation anti-infectives designed to address evolving bacterial drug resistance. The autoinducer-2 (AI-2) is a key quorum-sensing signal molecule which regulates bacterial group behaviors and is recognized by many Gram-negative and Gram-positive bacteria. Here

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