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Merck
CN

43314

TOS-propionate agar medium (acc. ISO)

NutriSelect® Plus, powder, for Bifidobacterium Spp., pack of 500 g

Synonym(s):

BSC Propionate Agar Base, Bifidobacteria Selective Count Agar Base, TOS-MUP Agar (Base), Transgalctosylated oligiosaccharide agar medium

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171609
Manufacturer/tradename:
NutriSelect® Plus
Agency:
IDF 220, according to ISO 29981
Form:
powder
Suitability:
Bifidobacterium Spp.
Application(s):
food and beverages
microbiology

Key Documents

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agency

IDF 220, according to ISO 29981

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L , ammonium sulfate, 3 g/L , casein enzymic hydrolysate, 10 g/L , L-cysteine hydrochloride, monohydrate, 0.5 g/L , dipotassium hydrogen phosphate, 4.8 g/L , Galactooligosaccharide, 10 g/L , magnesium sulfate heptahydrate, 0.2 g/L , Potassium dihydrogen phosphate, 3 g/L , sodium propionate, 15 g/L , yeast extract, 1 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

6.3±0.2 (25 °C)

application(s)

food and beverages
microbiology

suitability

Bifidobacterium Spp.

Quality Level

200

Related Categories

General description

TOS-propionate agar medium is specifically prepared for selective enumeration of Bifidobacteria in dairy products. Casein hydrolysate and yeast extract provide essential sources of nitrogen and other growth nutrients like vitamin B complex. The medium contains highly purified Galacto-oligosaccharides and sodium propionate that acts as an excellent growth promoting substances for Bifidobacteria. Cysteine hydrochloride helps in creating reduced conditions required for the growth. Ammonium sulphate and magnesium sulphate are a source of divalent cation, nitrogen, and sulphate. Lithium mupirocin is an antimicrobial supplement that inhibits the growth of most lactic acid bacteria, commonly found in fermented and non-fermented dairy products

Application

TOS-propionate agar medium is recommended for enumeration of presumptive Bifidobacteria by colony count technique from milk products.

Preparation Note

Suspend 62.02 grams of dehydrated media in 950 ml distilled water (1 liter fornon-selective media). Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 115°C for 15 minutes.
For selective isolation of Bifidobacteria add contents of 2 x 25 ml vials of Lithium mupirocin Supplement (Cat. No. 69732). Mix carefully to avoid the formation of air bubbles and dispense as desired.
Note: This medium being sensitive to heat, excessive heat treatment may therefore indicatively influence the properties of the medium. For more selectivity Glacial acetic acid (Bifido Selective Supplement B, Cat. No. 90577) may also be added. After addition of Bifido Selective Supplement B pH of the medium will shift to the acidic side, which does not affect the performance of the medium.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCP3080
BCCN2052
BCCL9929
BCCK3271
BCCJ4443

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S N Thitaram et al.
Letters in applied microbiology, 41(4), 355-360 (2005-09-16)
To determine the efficacy and selectivity of an acidified, antibiotic-selective, oligosaccharide-containing media for enumerating Bifidobacterium spp. from chicken caeca samples. Transoligosaccharide propionate agar medium (TOS) modified by addition of mupirocin (50 microg ml-1) and glacial acetic acid (1%, v/v), did
D Nyasha Chagwedera et al.
Cell metabolism, 30(2), 364-373 (2019-05-28)
Microbial dysbiosis and inflammation are implicated in diet-induced obesity and insulin resistance. However, it is not known whether crosstalk between immunity and microbiota also regulates metabolic homeostasis in healthy animals. Here, we report that genetic deletion of tuberous sclerosis 1

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