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Merck
CN

44940

Triple Sugar Iron Agar

NutriSelect® Basic, suitable for microbiology

Synonym(s):

TSI Agar

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171606

Key Documents

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Product Name

Triple Sugar Iron Agar, NutriSelect® Basic, suitable for microbiology

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12 g/L
ferrous sulfate, 0.2 g/L
glucose, 1 g/L
lactose, 10 g/L
meat extract, 3 g/L
mixed peptone, 20 g/L
phenol red, 0.025 g/L
sodium chloride, 5 g/L
sodium thiosulfate, 0.3 g/L
sucrose, 10 g/L
yeast extract, 3 g/L

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbe id | utilization test: suitable
microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
pharmaceutical
water monitoring
microbiology

suitability

nonselective and differential for Citrobacter spp.
nonselective and differential for Escherichia coli
nonselective and differential for Klebsiella spp.
nonselective and differential for Proteus spp.
nonselective and differential for Salmonella spp.
nonselective and differential for Shigella spp.
nonselective and differential for coliforms
nonselective and differential for enterobacteriaceae

Quality Level

100

Application

For the identification of Enterobacteriaceae acc. to Sulkin and Willett (1940), modified acc. to Hajna (1945).

Other Notes

Salmonella screening studies
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Dissolve 64.5 g in 1 litre distilled water. Warm to dissolve, mix well and pour into tubes, sterilize by autoclaving at 121°C for 15 minutes. Allow the medium to set in sloped form.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCL1234
BCCJ6190
BCCG2611
BCBX8920
BCBV0638

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M. Cristina et al.
Journal of Microbiological Methods, 8, 227-227 (1988)
H.J. Kolmos et al.
Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology, 95, 85-85 null
Sulkin, E.S., et al.
The Journal of Laboratory and Clinical Medicine, 25, 649-649 (1940)
Triple-Sugar Iron Agar Medium for the Identification of the Intestinal Group of Bacteria.
A A Hajna
Journal of bacteriology, 49(5), 516-517 (1945-05-01)

Articles

Microbiological Control of Spices and Herbs

Microbiological assessment of bacteria found in spices, a potent source for food spoilage and pathogens.

Differentiation and ID Media for Salmonella

Salmonella, with 2,300+ serotypes, causes half of food-borne illnesses, often from dairy, poultry, and eggs.

Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

沙门氏菌分离和鉴定培养基

沙门氏菌污染是全球食源性疾病的第二大成因。控制沙门氏菌的暴发是食品监管机构、餐馆和整个食品工业的一项重要任务。沙门氏菌家族包括2300多种血清型的细菌，但其中有两种类型，即肠炎沙门氏菌和鼠伤寒沙门氏杆菌，约占所有人类感染的一半。大多数沙门氏菌的暴发可以追溯到乳制品、家禽和肉制品，但沙门氏菌几乎可以在任何食物上生长。鸡、鸡蛋及其衍生产品的风险特别高。

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