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Merck
CN

46345

Fiske-Subbarow reducer

Synonym(s):

Fiske-Subbarow reagent

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About This Item

UNSPSC Code:
12352302
NACRES:
NA.25
MDL number:
Technical Service
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Quality Level

General description

formulation of 1-amino-2-naphthol-4-sulfonic acid, sodium sulfite and bisulfite according to C.H. Fiske and Y. Subbarow, J.Biol.Chem. 66, 375 (1925)

Application

The Fiske-Subbarow assay is a colorimetric assay designed to measure levels of inorganic phosphate in biological samples. Fiske-Subbarow reducer reagent is used as the reducer component of the Fiske-Subbarow assay.


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Danger

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Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1

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Storage Class

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

危险化学品

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Eduardo H S Sousa et al.
Analytical biochemistry, 348(1), 57-63 (2005-11-18)
We describe the development of a rapid colorimetric assay for soluble guanylate cyclase (sGC) activity adapted for a 96-well microplate. The assay greatly decreases the analysis time and cost over traditional methodologies based on radio- and immunoassays and high-performance liquid
E Durward et al.
BioTechniques, 25(4), 608-612 (1998-10-30)
Methods for detecting and measuring the success of nucleic acid sequence amplifications can be developed by detecting the by-products of amplification procedures. One method includes the detection of inorganic phosphate (Pi) during or on completion of the PCR. The method
A Kyaw et al.
Analytical biochemistry, 145(2), 230-234 (1985-03-01)
A new colorimetric procedure is described for inorganic phosphate determination using the color reaction between inorganic phosphate and acidified ammonium molybdate in the presence of Triton X-100. The method is simple and specific, and produces results comparable with those of



Global Trade Item Number

SKUGTIN
46345-5G-F04061832355399
46345-25G-F04061832860251