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48900

Giemsa Stain

Modified Solution

Synonym(s):

Azure eosin methylene blue, Giemsa solution

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About This Item

CAS Number:
51811-82-6
UNSPSC Code:
41116121
PubChem Substance ID:
329757421
NACRES:
NA.25
EC Number:
200-659-6
MDL number:
MFCD00081642
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Product Name

Giemsa Stain, Modified Solution, (for the staining (of cellular blood components and blood parasites))

type

(for research use only)

Quality Segment

200

shelf life

limited shelf life

technique(s)

microbe id | staining: suitable

suitability

Chlamydia spp., Histoplasma spp., Plasmodium spp., Trichomonas spp., bacteria, protozoa, spirochetes

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1

InChI

1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H

InChI key

NALREUIWICQLPS-UHFFFAOYSA-N

Application

Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams & Wilkins, 173 (1981); together with May-Grünwald Solution for pap staining according to Pappenheim.

Biochem/physiol Actions

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Physical form

Solution of azure B/azure II-eosin/methylene blue 1:12:2 (w/w/w) in glycerol/methanol 5:24 (v/v); total dye content: 0.6 % (w/w)

Preparation Note

Fix 10 min. in methanol; air-dry; immerse 45 min. in Giemsa Solution; rinse with distilled water; air-dry

Disclaimer

store at 20°C

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wgk

WGK 2

Storage Class

3 - Flammable liquids

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

flash_point_f

53.6 °F

flash_point_c

12 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

target_organs

Eyes,Central nervous system

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