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Sigma-Aldrich

Methylene Blue solution

suitable for microbiology

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Synonym(s):
Ehrlich’s reagent III, Loeffler’s Methylene Blue
Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
61-73-4
Molecular Weight:
319.85
Beilstein:
3641570
MDL number:
MFCD00012111
PubChem Substance ID:
329757603
NACRES:
NA.47

shelf life

limited shelf life, expiry date on the label

concentration

0.5% in H2O (0.5 g/100 mL)

technique(s)

microbe id | staining: suitable

application(s)

microbiology

suitability

Bacillus spp.
Corynebacterium spp.
bacteria

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

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General description

Methylene Blue was earlier recognized as a good nuclear stain, the staining solutions are always either aged or alkalized, if not both. More importantly it is an excellent stain for ribonucleic acid, which is the important stainable constituent of bacterial bodies, of lymphocyte and hemoportoal cytoplasm, and of nerve cell tigroid granules. It can also be employed in capsule stain. The capsule stain begins as a negative stain leaving the organisms colorless. The capsule are clearly seen as halo surrounding the bacterial cell. Another staining procedure is performed with nigrosin (Cat. No. 43925).

Components

reagent contains:
methylene blue 0.5 g
distilled water 100 ml

replaced by

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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