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Merck
CN

51489

Rapid Coliform ChromoSelect Broth

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Coliform Rapid ChromoSelect Broth

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171606

Key Documents

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Product Name

Rapid Coliform ChromoSelect Broth, suitable for microbiology, NutriSelect® Plus

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

chromogenic substrate, 0.08 g/L
dipotassium hydrogen phosphate, 2.7 g/L
fluorogenic substrate, 0.05 g/L
IPTG, 0.1 g/L
peptone, special, 5 g/L
potassium dihydrogen phosphate, 2 g/L
sodium chloride, 5 g/L
sodium lauryl sulfate, 0.1 g/L
sorbitol, 1 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

6.8±0.3 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

storage temp.

2-8°C

suitability

selective and differential for Escherichia coli
selective and differential for coliforms

Quality Level

200

Related Categories

Application

Rapid Coliform ChromoSelect Broth is used for detection and conformation of Escherichia coli and coliforms on the basics of enzyme substrate reaction from water samples, using a combination of chromogenic and fluorogenic substrate.

Other Notes

Medium for the assay of water for Coliforms
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Suspend 16.0 g (single strength) or 32.0 g (double strength) in 1 litre distilled water. Boil to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 1 bar pressure (121 °C) for 15 minutes.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL5469
BCCD6203
BCBT8396
BCBS2398V

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G. Hahn, E. Wittrock
Acta Microbiologica Academiae Scientiarum Hungaricae, 38, 225-225 (1991)
M Manafi et al.
Microbiological reviews, 55(3), 335-348 (1991-09-01)
Methods based on the application of chromogenic and fluorogenic substrates enable specific and rapid detection of a variety of bacterial enzymatic activities. By using these techniques, enzymatic reactions can be examined simultaneously or individually, either directly on the isolation plate

Articles

Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

显色培养基概述

选择性培养基可以为微生物的检测、识别和计数提供更快的结果和肉眼确认

用于分化和检测大肠杆菌和其他大肠菌群的选择性生长培养基

有关用于分化和检测大肠杆菌和其他大肠菌群的选择性生长培养基的一篇文章。

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