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Sigma-Aldrich

Ethanol

BioUltra, for molecular biology, ≥99.8%, (absolute alcohol, without additive, A15 o1)

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Synonym(s):
Ethyl alcohol
Linear Formula:
CH3CH2OH
CAS Number:
64-17-5
Molecular Weight:
46.07
Beilstein:
1718733
EC Number:
200-578-6
MDL number:
MFCD00003568
PubChem Substance ID:
329757771
NACRES:
NA.25

grade

for molecular biology

Quality Level

200

vapor density

1.59 (vs air)

vapor pressure

44.6 mmHg ( 20 °C)

product line

BioUltra

Assay

≥99.8%

form

liquid

autoignition temp.

683 °F

quality

(absolute alcohol, without additive, A15 o1)

expl. lim.

19 %, 60 °F

impurities

DNases, none detected
 KMnO4-reducing substances, in accordance
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected
 substances darkened by H2SO4, in accordance
≤0.0004% free alkali (as NH3)
≤0.001% aldehyde (as CH3CHO)
≤0.001% formaldehyde (as HCHO)
≤0.001% ketone (as CH3COCH3)
≤0.002% free acid (as CH3COOH)
≤0.1% methanol (CH3OH)
≤0.2% water

evapn. residue

≤0.001%

refractive index

n20/D 1.3600 (lit.)
n20/D 1.362

bp

78 °C (lit.)

mp

−114 °C (lit.)

solubility

H2O: miscible
organic solvents: miscible

density

0.789 g/mL at 25 °C (lit.)

cation traces

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.1 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤1 mg/kg
Ni: ≤0.02 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

SMILES string

CCO

λ

neat

UV absorption

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.03

suitability

suitable for molecular biology

application(s)

life science and biopharma

format

neat

InChI

1S/C2H6O/c1-2-3/h3H,2H2,1H3

InChI key

LFQSCWFLJHTTHZ-UHFFFAOYSA-N

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General description

Ethanol, also known as Ethyl Alcohol, is a volatile primary alcohol with a straight-chain structure. It is frequently employed as a biochemical reagent, effectively denaturing proteins from bacteria, fungi, and viruses. Widely utilized in cell biology, molecular biology, and biochemical research, this DNase, RNase, NICKase, and protease-free form of ethanol plays a crucial role in purifying and precipitating biomolecules like nucleic acids (DNA & RNA) and proteins. Additionally, it finds application in histology for preparing staining and destaining reagents, as well as in the dehydration of tissues before embedding.

Application

Ethanol has been used:
  • in vehicle-treated controls to perform mouse experiments
  • in viability assay along with crystal violet to stain human and mouse high-grade serous ovarian cancer (HGSOC) cells after stimulation
  • to prepare 17β-estradiol (E2) to use in cell culture experiments
  • in cell cycle analysis for fixing cells drop-wise

Biochem/physiol Actions

Ethanol is a widely used organic chemical in industry and consumer goods. This aliphatic alcohol is mainly used in industry as a solvent and as an intermediate in the production of other chemicals.

Features and Benefits

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • BioUltra Grade for your Molecular Biology, Cell Biology and Biochemical research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Free from DNase, RNase, NICKase and proteaseTested for trace levels of Anions and Cations

Other Notes

For additional information on our range of Biochemicals, please complete this form.
Sales restrictions may apply. Suitable for the precipitation of nucleic acids

Footnote

1Distribution and use of ethyl alcohol in Switzerland require a license from the Swiss Federal Alcohol Administration

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Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H225 - H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

55.4 °F

Flash Point(C)

13 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品

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G M Huang et al.
Analytical biochemistry, 223(1), 35-38 (1994-11-15)
Genome projects require a high-throughput method for DNA template preparation, which traditional protocols can rarely provide. We introduce here a new protocol for plasmid and cosmid DNA preparation based on alkaline denaturation in a 96-well format. The essential improvement made
M G Mahoney et al.
The Journal of clinical investigation, 103(4), 461-468 (1999-02-18)
Patients with pemphigus foliaceus (PF) have blisters on skin, but not mucous membranes, whereas patients with pemphigus vulgaris (PV) develop blisters on mucous membranes and/or skin. PF and PV blisters are due to loss of keratinocyte cell-cell adhesion in the
D M Lovinger et al.
Science (New York, N.Y.), 243(4899), 1721-1724 (1989-03-31)
The ion current induced by the glutamate receptor agonist N-methyl-D-aspartate (NMDA) in voltage-clamped hippocampal neurons was inhibited by ethanol (EtOH). Inhibition increased in a concentration-dependent manner over the range 5 to 50 mM, a range that also produces intoxication. The
Preparation and properties of serum and plasma proteins; crystallization of serum albumins from ethanol water mixtures.
E J COHN et al.
Journal of the American Chemical Society, 69(7), 1753-1761 (1947-07-01)
Y Gavrieli et al.
The Journal of cell biology, 119(3), 493-501 (1992-11-01)
Programmed cell death (PCD) plays a key role in developmental biology and in maintenance of the steady state in continuously renewing tissues. Currently, its existence is inferred mainly from gel electrophoresis of a pooled DNA extract as PCD was shown
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Solvent Miscibility Table

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