Skip to Content
Merck
CN

53189

Mannitol Lysine Crystal Violet Brilliant Green Agar

NutriSelect® Plus, powder, nonselective and differential for Salmonella spp., pack of 500 g, suitable for microbiology

Synonym(s):

MLCB AGAR, Mannitol Lysine Agar

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

NACRES:
NA.85
UNSPSC Code:
41171606
Manufacturer/tradename:
NutriSelect® Plus
Form:
powder
Suitability:
nonselective and differential for Salmonella spp.
Application(s):
clinical testing
food and beverages
veterinary, microbiology

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

sterility

non-sterile

form

powder

Quality Level

200

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L , beef extract, 2.0 g , briliant green, 0.0125 g/L , crystal violet, 0.010 g/L , ferric ammonium citrate, 1.0 g/L , L-lysine hydrochloride, 5.0 g/L , mannitol, 3.0 g/L , peptic digest of animal tissue, 10.0 g/L , sodium chloride, 4.0 g/L , sodium thiosulfate, 4.0 g/L , yeast extract, 5.0 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

6.8±0.2 (25 °C)

application(s)

clinical testing
food and beverages
veterinary, microbiology

suitability

nonselective and differential for Salmonella spp.

Related Categories

General description

Mannitol Lysine Crystal Violet Brilliant Green Agar (MLCB Agar) is formulated for the selective isolation of Salmonella species, excluding Salmonella typhi and Salmonella paratyphi A, from fecal and food products. The medium contains peptic digest of animal tissue, beef extract, and yeast extract, which provides essential nutrients to support the growth of Salmonella. Mannitol serves as the fermentable carbohydrate, enabling the detection of fermentation activity, while L-lysine acts as the amino acid that facilitates lysine decarboxylation, a key metabolic process in Salmonella identification. This selective medium is designed to enhance the isolation and differentiation of Salmonella species in complex sample matrices.

Application

Mannitol Lysine Crystal Violet Brilliant Green Agar is used for isolating Salmonella species, excluding Salmonella typhi and Salmonella paratyphi A, from fecal samples and food products. It is widely applied in clinical testing for disease diagnosis, the food and beverage industry for ensuring microbiological safety, veterinary testing for disease control in animals, and microbiology research for studying Salmonella behavior in various environments.

Features and Benefits

NutriSelect® Plus is a powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

Preparation Note

Suspend 49.02 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. DO NOT AUTOCLAVE. Cool to 45-50°C. Mix well and pour into sterile Petri plates.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Salmonellae grow as large purple-black colonies with black centers due to hydrogen sulfide production, facilitated by sodium thiosulfate and ferric ammonium citrate. Mannitol fermentation produces acid, stimulating lysine decarboxylation, which elevates the pH and promotes blackening. Atypical Salmonella strains producing little or no hydrogen sulfide may appear as mauve-grey colonies with a central black "bull′s eye." Mannitol Lysine Medium can be directly inoculated or used after enrichment in Modified Semisolid RV Medium Base. For better detection of atypical strains, Brilliant Green Agar (Modified) or Bismuth Sulfite Agar should be used alongside Mannitol Lysine Medium.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

hcodes

H412

Hazard Classifications

Aquatic Chronic 3

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

新产品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCP3025
BCCM7837
BCCL5439
BCCK8198
BCCK4947

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service