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Merck
CN

556416

New Methylene Blue N

Dye content ~70 %

Synonym(s):

Basic Blue 24, Methylene Blue N

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About This Item

Empirical Formula (Hill Notation):
C18H22ClN3S
CAS Number:
1934-16-3
Molecular Weight:
347.91
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24879556
EC Number:
217-696-9
Colour Index Number:
52030
MDL number:
MFCD00135878

Key Documents

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Product Name

New Methylene Blue N, Dye content ~70 %

InChI key

NZYCYASKVWSANA-UHFFFAOYSA-M

InChI

1S/C18H22N3S.ClH/c1-5-19-13-9-17-15(7-11(13)3)21-16-8-12(4)14(20-6-2)10-18(16)22-17;/h7-10,19-20H,5-6H2,1-4H3;1H/q+1;/p-1

SMILES string

[Cl-].CCNc1cc2[s+]c3cc(NCC)c(C)cc3nc2cc1C

form

powder, crystals or chunks

composition

Dye content, ~70%

technique(s)

microbe id | staining: suitable

mp

190 °C (dec.) (lit.)

λmax

590 nm
632 nm (2nd)

εmax

≥29000 at 626-634 nm in water
≥30500 at 586-594 nm in water

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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Application

New methylene blue N has been used as a photosensitizer for the photodynamic inactivation of Candida albicans.

Biochem/physiol Actions

New methylene blue N can bind, neutralize and crosslink with RNA. This causes co-precipitation of ribosomal and residual RNA with the mitochondria and ferritin masses. These events make the living young erythrocytes as microscopically visible, dark-blue clusters and filaments, referred to as reticulum. New Methylene Blue N helps in counting of reticulocytes (erythrocytes containing RNA).

Features and Benefits

Solvent soluble cationic thiazine dye

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000376807
0000376807
0000164445
0000164445
SHBN9708

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J O'Neill et al.
Journal of chemotherapy (Florence, Italy), 15(4), 329-334 (2003-09-10)
In order to establish a comparative order of efficacy among established photosensitising compounds currently under investigation, the in vitro photobactericidal activities of six commercially available photosensitisers were investigated at equal concentration against Streptococcus sanguis using a Helium Neon (HeNe) laser
Jennifer L Lansdowne et al.
American journal of veterinary research, 66(8), 1324-1329 (2005-09-22)
To determine the relationship between epidural cranial migration and injectate volume of an isotonic solution containing dye in laterally recumbent foal cadavers and evaluate the cranial migration and dermatome analgesia of an epidural dye solution during conditions of laparoscopy in
Diego A Portela et al.
Veterinary anaesthesia and analgesia, 39(4), 398-408 (2012-05-31)
To describe the landmarks and methodology to approach the thoracic paravertebral space in dogs; to evaluate if intercostal muscular response could be evoked by a nerve-stimulator; to radiographically assess the distribution pattern of a radio-opaque contrast medium after thoracic paravertebral
M Shaffer et al.
Pediatric pathology, 13(5), 591-595 (1993-09-01)
Although reticulocyte counts can be reliably performed for up to 48 h after storage in EDTA, it is unclear whether this is applicable to the pediatric age group. In order to evaluate this, manual reticulocyte counts were performed on 20
M J Lopez et al.
American journal of veterinary research, 58(7), 786-790 (1997-07-01)
To determine the relation between epidural injectate volume (ml/kg of body weight) and its craniad migration in calves and pigs. 23 neonatal calves and 26 feeder pigs. Animals were randomly assigned to receive different volumes of new methylene blue (NMB
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