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Millipore

M-17 Broth

NutriSelect® Basic, powder, for Streptococcus thermophilus, pack of 500 g

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.85

sterility

non-sterile

Quality Level

100

form

powder

shelf life

limited shelf life, expiry date on the label

composition

ascorbic acid, 0.5 g/L
lactose, 5 g/L
magnesium sulfate, 0.25 g/L
meat extract, 5 g/L
meat peptone (peptic), 2.5 g/L
sodium glycerophosphate, 19 g/L
soya peptone (papainic), 5 g/L
tryptone, 2.5 g/L
yeast extract, 2.5 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

pH

7.0±0.2 (25 °C)

application(s)

food and beverages
microbiology

storage temp.

2-25°C

suitability

Streptococcus thermophilus
nonselective for Enterococcus spp.
nonselective for Staphylococcus spp.

General description

M-17 Broth is a selective medium for Streptococcus thermophilus in the common examination of yoghurt. It is superior to other comparable culture media for the cultivation of the fastidious species such as S. cremoris, S. diacetilactis and S. lactis. Mutants which are incapable of metabolizing lactose can also be isolated on these media. Tryptone, meat peptone, soya peptone, yeast extract and meat extract provide carbonaceous, nitrogenous compounds, vitamin B complex and other essential growth factors. Lactose is the fermentable carbohydrate and ascorbic acid is stimulatory for the growth of lactic Streptococci. Magnesium sulphate provides essential ions to the organisms. Sodium glycerophosphate increases the buffering capacity of the medium, this promotes the growth of lactic streptococci and inhibits lactobacilli growth.

Application

M-17 Broth is a selective medium for Streptococcus thermophilus for examination of yogurt and for the enrichment Streptococcus thermophilus in the dairy industry.

Preparation Note

Suspend 42 g of powder in 1 litre of distilled water and let it soak. Heat to boiling and dispense into suitable containers. Sterilize by autoclaving at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCN0467
BCCN0466
BCCM3452
BCCL5378
BCCL3831

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