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Lectin, immobilized on Agarose CL-4B from Triticum vulgaris

suitable for affinity chromatography

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Synonym(s):
Wheat germ agglutinin, immobilized on agarose CL-4B
NACRES:
NA.21

technique(s)

affinity chromatography: suitable

Quality Level

capacity

0.1-1.0 mg/mL, packed gel capacity (ovomucoid)

storage temp.

2-8°C

Physical form

50% aqueous suspension in 1 M NaCl

Other Notes

Protein isolation from bordetella pertussis that facilitates entry of calmodulin-sensitive adenylate cyclase into animal cells

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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M G Donovan et al.
Biochemistry, 28(20), 8124-8129 (1989-10-03)
Bordetella pertussis, the pathogen responsible for whooping cough, releases a soluble calmodulin-sensitive adenylate cyclase into its culture medium. Several investigators have shown that the partially purified adenylate cyclase is capable of entering animal cells and elevating intracellular cAMP levels [Confer

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Protocols

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This page shows how to prepare samples for purification with affinity chromatography.

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