63184
5-Maleimido-eosin
suitable for fluorescence, ≥93% (HPLC)
Synonym(s):
EMA, Eosin-5-maleimide
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About This Item
Empirical Formula (Hill Notation):
C24H9Br4NO7
CAS Number:
Molecular Weight:
742.95
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352207
MDL number:
Quality Level
assay
≥93% (HPLC)
form
powder
mol wt
742.95 g/mol
color
light orange to dark orange, light red
fluorescence
λex 524 nm; λem 545 nm (after derivatization with 2-mercaptoethanol)
suitability
suitable for fluorescence
storage temp.
2-8°C
SMILES string
Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5cc(ccc45)N6C(=O)C=CC6=O)c1Br
InChI
1S/C24H9Br4NO7/c25-13-6-11-21(17(27)19(13)32)35-22-12(7-14(26)20(33)18(22)28)24(11)10-2-1-8(5-9(10)23(34)36-24)29-15(30)3-4-16(29)31/h1-7,32-33H
InChI key
YRCLWXRIEYQTAV-UHFFFAOYSA-N
General description
5-Maleimido-eosin, also known as EMA or Eosin-5-maleimide, is a fluorescent binding agent. The 5-Maleimido-eosin binding test measures the EMA-labeled cells reduced mean channel fluorescence (MCF) reading.
Application
Eosin-5-maleimide has been used as a fluorescent probe for the human erythrocyte band 3 protein. It is widely used as a diagnostic probe for the laboratory detection of hereditary spherocytosis (HS) by flow cytometry. The eosin-5-maleimide binding test was done to analyze the effect of alectinib (first line of treatment) for anaplastic lymphoma kinase (ALK).
Other Notes
Triplet probe. Measurement of rotational diffusion of protein in solution and in membranes, including myosin and pyruvate dehydrogenase
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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S Ishiwata et al.
The Journal of biological chemistry, 262(17), 8314-8317 (1987-06-15)
We studied the rotational Brownian motions of myosin heads, of which the sulfhydryl group was selectively labeled with the triplet probe 5-eosinylmaleimide, in myofibril by using flash-induced phosphorescence anisotropy decay measurements. The anisotropy decay curve under relaxing conditions consisted of
Linda Hunt et al.
Cytometry. Part B, Clinical cytometry, 88(1), 50-57 (2014-09-18)
The eosin-5'-maleimide (EMA) Binding test measures reduced mean channel fluorescence (MCF) reading of EMA-labeled red cells (EMA-RBCs) from patients with hereditary spherocytosis (HS). Reporting test results can be either in the actual MCF reading or as a ratio by normalization
Renée L Crisp et al.
Pediatric blood & cancer, 59(7), 1299-1301 (2012-04-11)
We studied 31 children with hemolytic anemia, or with positive family history for hereditary spherocytosis (HS), to assess the reliability of capillary blood samples for the diagnosis. HS was diagnosed in 20 patients. Cryohemolysis (CH) was positive in 94% and
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A3412-5G | 04061832489384 |
| A3412-10G | 04061833360835 |
| A3412-1G | 04061833360842 |
| 63184-10MG | 04061832721255 |
| 63184-25MG | 04061833277751 |