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63184

5-Maleimido-eosin

suitable for fluorescence, ≥93% (HPLC)

Synonym(s):

EMA, Eosin-5-maleimide

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About This Item

Empirical Formula (Hill Notation):
C24H9Br4NO7
CAS Number:
150322-02-4
Molecular Weight:
742.95
NACRES:
NA.32
PubChem Substance ID:
329759808
UNSPSC Code:
12352207
MDL number:
MFCD07437936
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Quality Segment

assay

≥93% (HPLC)

form

powder

mol wt

742.95 g/mol

technique(s)

protein detection: suitable

color

light orange to dark orange, light red

fluorescence

λex 524 nm; λem 545 nm (after derivatization with 2-mercaptoethanol)

suitability

suitable for fluorescence

storage temp.

2-8°C

SMILES string

Oc1c(Br)cc2c(Oc3c(Br)c(O)c(Br)cc3C24OC(=O)c5cc(ccc45)N6C(=O)C=CC6=O)c1Br

InChI

1S/C24H9Br4NO7/c25-13-6-11-21(17(27)19(13)32)35-22-12(7-14(26)20(33)18(22)28)24(11)10-2-1-8(5-9(10)23(34)36-24)29-15(30)3-4-16(29)31/h1-7,32-33H

InChI key

YRCLWXRIEYQTAV-UHFFFAOYSA-N

General description

5-Maleimido-eosin, also known as EMA or Eosin-5-maleimide, is a fluorescent binding agent. The 5-Maleimido-eosin binding test measures the EMA-labeled cells reduced mean channel fluorescence (MCF) reading.

Application

Eosin-5-maleimide has been used as a fluorescent probe for the human erythrocyte band 3 protein. It is widely used as a diagnostic probe for the laboratory detection of hereditary spherocytosis (HS) by flow cytometry. The eosin-5-maleimide binding test was done to analyze the effect of alectinib (first line of treatment) for anaplastic lymphoma kinase (ALK).

Other Notes

Triplet probe. Measurement of rotational diffusion of protein in solution and in membranes, including myosin and pyruvate dehydrogenase

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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