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66720

Sigma-Aldrich

Methylene Blue hydrate

purum p.a., for microscopy (Bact., Bot., Hist., Vit.), ≥97.0% (calc. based on dry substance, AT)

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Empirical Formula (Hill Notation):
C16H18ClN3S · xH2O
CAS Number:
Molecular Weight:
319.85 (anhydrous basis)
Beilstein:
3599847
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

grade

for microscopy (Bact., Bot., Hist., Vit.)
purum p.a.

Quality Level

Assay

≥97.0% (calc. based on dry substance, AT)

SMILES string

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

InChI key

WQVSELLRAGBDLX-UHFFFAOYSA-M

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General description

Methylene Blue (MB) is a basic histological dye.

Application

Methylene Blue hydrate has been used:
  • in the preparation of methylene blue (MB) solution to stain zymograms
  • to label the injection site in the tissue slices
  • to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss

Biochem/physiol Actions

Methylene blue (MB) plays a key role in microbiology and pharmacology. It is mainly used in staining live organisms and in methemoglobinemia treatment. MB can be considered as a possible and safe vasoconstrictor. It has several applications in textile, paper and hair coloring sectors.

Other Notes

may contain up to 22% water
On the efficiency of methylene blue versus persulfate catalysis of polyacrylamide gels, as investigated by capillary zone electrophoresis

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Methylene blue in photodynamic therapy: from basic mechanisms to clinical applications
Tardivo Jp, et al.
Photodiagnosis and Photodynamic Therapy, 2(3), 175-191 (2005)
Methylene blue in the treatment of vasodilatory shock: a Meta-analysis
Zhang X, et al.
Zhonghua Wei Zhong Bing ji Jiu yi Xue, 2(3), 175-191 (2017)
Efficient removal of different basic dyes using graphene
Iqbala MZ, et al.
Desalination and Water Treatment, 68(3), 226-235 (2017)
UDP-Induced Phagocytosis and ATP-Stimulated Chemotactic Migration Are Impaired in STIM1-/- Microglia In Vitro and In Vivo
Lim HM, et al.
Mediators of Inflammation, 2017(3), e1687-e1687 (2017)
Attenuation of noise-induced hearing loss using methylene blue
Park JS, et al.
Cell Death & Disease, 5(4), e1200-e1200 (2014)

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