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Merck
CN

66720

Methylene Blue hydrate

purum p.a., suitable for microscopy, ≥97.0% (calc. based on dry substance, AT)

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S · xH2O
CAS Number:
122965-43-9
Molecular Weight:
319.85 (anhydrous basis)
UNSPSC Code:
12171500
NACRES:
NA.32
PubChem Substance ID:
329760310
EC Number:
200-515-2
Beilstein/REAXYS Number:
3599847
MDL number:
MFCD00150006

Key Documents

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Product Name

Methylene Blue hydrate, purum p.a., suitable for microscopy, ≥97.0% (calc. based on dry substance, AT)

InChI key

WQVSELLRAGBDLX-UHFFFAOYSA-M

InChI

1S/C16H18N3S.ClH.H2O/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;;/h5-10H,1-4H3;1H;1H2/q+1;;/p-1

SMILES string

O.[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

grade

purum p.a.

assay

≥97.0% (calc. based on dry substance, AT)

Quality Level

200

λmax

656-666 nm in water

form

powder

mol wt

319.85 g/mol (anhydrous basis)

concentration

≤100% (Methylthioninium chloride hydrate)

color

green to dark green

solubility

ethanol: 0.01 g/10 mL, blue to very deep blue

suitability

suitable for microscopy

storage temp.

room temp

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Application

Methylene Blue hydrate has been used:
  • in the preparation of methylene blue (MB) solution to stain zymograms
  • to label the injection site in the tissue slices
  • to pre-treat mice via oral gavage to study its effects on noise-induced hearing loss

Biochem/physiol Actions

Methylene blue (MB) plays a key role in microbiology and pharmacology. It is mainly used in staining live organisms and in methemoglobinemia treatment. MB can be considered as a possible and safe vasoconstrictor. It has several applications in textile, paper and hair coloring sectors.

General description

Methylene Blue (MB) is a basic histological dye.

Other Notes

On the efficiency of methylene blue versus persulfate catalysis of polyacrylamide gels, as investigated by capillary zone electrophoresis
may contain up to 22% water

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCN5573
BCCN1625
BCCN0560
BCCM4231
BCCH3435

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Preparation and analysis of crude autolytic enzyme extracts from Staphylococcus aureus
Vaz F, et al.
Bio-protocol, 5(3), e1687-e1687 (2015)
Attenuation of noise-induced hearing loss using methylene blue
Park JS, et al.
Cell Death & Disease, 5(4), e1200-e1200 (2014)
S Caglio et al.
Electrophoresis, 14(10), 997-1003 (1993-10-01)
The efficiency of a novel method of photopolymerization, based on photoinitiating the reaction with methylene blue (MB), in presence of a redox couple (sodium toluenesulfinate and diphenyliodonium chloride), vs, the conventional persulfate--N,N,N',N'-tetramethylethylenediamine redox couple was investigated as a function of
Efficient removal of different basic dyes using graphene
Iqbala MZ, et al.
Desalination and Water Treatment, 68(3), 226-235 (2017)
Methylene blue in the treatment of vasodilatory shock: a Meta-analysis
Zhang X, et al.
Zhonghua Wei Zhong Bing ji Jiu yi Xue, 2(3), 175-191 (2017)
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