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Merck
CN

66725

Methylene Blue solution alkaline according to Loeffler

according to Loeffler

Synonym(s):

Methylene Blue solution, Ehrlich’s reagent III, Loeffler’s Methylene Blue

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S
CAS Number:
Molecular Weight:
319.85
UNSPSC Code:
12352200
NACRES:
NA.25
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3641570
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InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

shelf life

limited shelf life, expiry date on the label

composition

dye (C.I.No. 52015), 0.3 % (w/v) (in ethanol:0.1% aqueous KOH 3:10)

refractive index

n20/D 1.347

Quality Level

Application

Loeffler′s alkaline methylene blue stain solution has been used for the staining of microalgae cells.

Other Notes

Prepared according to Clark

pictograms

Flame

signalword

Warning

hcodes

Hazard Classifications

Flam. Liq. 3

Storage Class

3 - Flammable liquids

wgk

WGK 2

flash_point_f

84.2 °F - closed cup

flash_point_c

29 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Regulatory Information

危险化学品
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G. Clark
Staining Procedures, 380-380 (1981)
Associated effects of storage and mechanical pre-treatments of microalgae biomass on biomethane yields in anaerobic digestion.
Gruber-Brunhumer MR et al.
Biomass and Bioenergy, 93, 259-268 (2016)
Jonel Steffen et al.
The British journal of ophthalmology, 98(1), 13-15 (2013-10-26)
To evaluate the diagnostic accuracy of methylene blue used as a non-invasive in vivo stain to detect ocular surface squamous neoplasia (OSSN). A test validation study was performed according to Standards for the reporting of diagnostic accuracy studies (STARD) guidelines
Q R J G Tummers et al.
European journal of surgical oncology : the journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology, 40(7), 850-858 (2014-05-28)
Despite recent developments in preoperative breast cancer imaging, intraoperative localization of tumor tissue can be challenging, resulting in tumor-positive resection margins during breast conserving surgery. Based on certain physicochemical similarities between Technetium((99m)Tc)-sestamibi (MIBI), an SPECT radiodiagnostic with a sensitivity of
Nicolaas Lucius Gerlach et al.
Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 72(4), 666-671 (2014-02-01)
To establish the accuracy of cone-beam computed tomographic (CBCT) views in determining the position and diameter of the mandibular canal. Two freshly frozen cadaver heads, 1 dentate and 1 edentate, were used to acquire CBCT scans. Measurements on cross-sectional CBCT

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