67447
STEC Millichrome™ plus Agar Base
for bacteria: Escherichia coli (Shiga toxin STEC), suitable for microbiology
Synonym(s):
Agar Base for STEC, Chromogenic Agar Base for STEC Detection, STEC Detection and Identification Agar Base
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About This Item
NACRES:
NA.74
UNSPSC Code:
41161501
Quality Segment
agency
EP, JP, USP
sterilization compatibility
dry heat compatible
form
dry powder
shelf life
limited shelf life, expiry date on the label
composition
Agar, 15.0 g/L , Chromogenic Mix, 2.6 g/L , Peptone & Yeast extract, 8.0 g/L , Salts, 5.2 g/L
manufacturer/tradename
MilliChrome™
technique(s)
microbiological culture: suitable
final pH
6.0±0.2 (25 °C)
solubility
deionized water: 30.8 g/L
application(s)
food and beverages, microbiology
shipped in
ambient
storage temp.
15-25°C
suitability
bacteria: Escherichia coli (Shiga toxin STEC)
General description
STEC Millichrome™ plus Agar Base is a selective chromogenic culture medium used for the identification of Shiga-like toxin-producing E. coli (STEC). The media contains chromogenic substrates that produce visible color changes upon enzymatic activity of E. coli. Peptone and yeast extract are nitrogenous nutrients for growth and other essential growth factors. Sodium chloride provides osmotic balance. The chromogenic mix contains chromogenic substrates for color differentiation based on the ability to cleave the substrate by characteristic enzymes. STEC Millichrome™ plus supplement contains selective agents that allow STEC to grow while other organisms are inhibited. Agar is a solidifying agent.
Application
STEC Millichrome™ plus Agar Base is a highly selective chromogenic culture medium intended for use in the qualitative direct detection, differentiation, and presumptive identification of Shiga-like-toxin producing E. coli (STEC) in various samples, including food products for human consumption, animal feed, and environmental samples.
Preparation Note
- Store prepared media below 8°C, protected from direct light (max. 1 month).
- Store dehydrated powder, in a dry place, in tightly sealed containers at 2-25°C.
- The rehydrated supplement is stable for up to 2 months when stored at 2-8°C
Step 1 (Preparation of the base STEC Millichrome™ plus Agar Base)
- Disperse slowly 30.8 g of powder base in 1 L of purified water.
- Stir until agar is well thickened.
- Heat and bring to boil (100 °C) while swirling or stirring regularly. DO NOT HEAT TO MORE THAN 100 °C. DO NOT AUTOCLAVE AT 121 °C.
- Cool in a water bath to 45-50 °C. Swirl or stir gently to homogenize.
- Aseptically rehydrate 1 vial with 10 mL of sterile water.
- Swirl well until complete dissolution.
- Add 1 vial rehydrated solution to 1L STEC Millichrome™ plus Agar Base cooled at 45-50 °C.
- Swirl gently to homogenize.
- Pour into sterile Petri dishes
- Let it solidify and dry.
Analysis Note
The presence of mauve colonies indicates the presence of STEC. Other organisms may show colorless or blue colonies or be inhibited. STEC O157 is nonfluorescent under UV light, while other STEC serotypes are variable in fluorescence under the UV lamp at 365nm.
Legal Information
MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
The product is notintended for diagnosing infections or guiding treatment.
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
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