STEC Millichrome^™ plus Supplement

STEC Millichrome^™ plus Supplement is a selective media supplement for the detection of E. coli (STEC). The distinguishing feature of these media is the inclusion of a chromogenic substrate mix that produces visible color changes upon enzymatic activity of E. coli (STEC).Millichrome^™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The package contains 5 vials, each vial sufficient for 1 liter of final media. One vial of the supplement should be rehydrated with 10 mL of sterile water and then added to 1 liter of melted STEC Millichrome^™ plus Agar base cooled at 45-50 °C.

STEC Millichrome^™ plus Supplement is a selective chromogenic media supplement intended for use in the qualitative detection, differentiation and presumptive identification of E. coli (STEC) from food, animal feed, and environmental samples.

5 vials (1 vial is sufficient for 1 L of final media)

The Shiga toxins E. coli is detected by a characteristic mauve color after only 24 h at an optimal growth temperature of 37°C, while most other Enterobacteriaceae form Colorless, blue, or inhibited after incubation at the same temperature

MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany