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Merck
CN

69964

MRS Agar

NutriSelect® Basic, powder, nonselective for Lactobacillus Spp., pack of 500 g

Synonym(s):

Lactobacillus Agar according to DeMan, Rogosa and Sharpe, Lactobacillus Agar according to DeMan, Rogosa, and Sharpe

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About This Item

UNSPSC Code:
41171605
NACRES:
NA.74

Key Documents

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sterility

non-sterile

Quality Level

100

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 12 g/L
dipotassium hydrogen phosphate, 2 g/L
D(+)-glucose, 20 g/L
magnesium sulfate (equivalent to 0.2 g/lheptahydrate), 0.1 g/L
manganous sulfate monhydrate, 0.05 g/L
meat extract, 5 g/L
sodium acetate, 5 g/L
triammonium citrate, 2 g/L
universal peptone, 10 g/L
yeast extract, 5 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Basic

technique(s)

microbiological culture: suitable

final pH

6.5±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
microbiology

suitability

nonselective for Lactobacillus Spp.
selective by low pH for Bifidobacterium spp.

Related Categories

General description

MRS Agar, also known as DeMan-Rogosa-Sharpe Agar, is a microbiological medium specifically designed for the enrichment, cultivation, and isolation of Lactobacillus species from various materials. It is a non-selective medium with a low pH that favors the growth of Lactobacillus spp. and Bifidobacterium spp. The medium contains essential nutrients such as glucose, meat extract, yeast extract, and peptones, which provide nitrogenous and carbonaceous compounds, amino acids, and vitamins necessary for microbial growth. Sodium acetate and ammonium citrate enhance selectivity by inhibiting the growth of unwanted organisms. The media contains polysorbate (Tween 80), acetate, magnesium, and manganese, which are known to act as specific growth factors for Lactobacilli, and provide a rich nutrient base.

Application

MRS Agar is widely used for the detection, isolation, and cultivation of Lactobacillus spp. and other lactic acid bacteria from food, beverages, and environmental samples. It is applied in agriculture, food and beverage quality control, environmental monitoring, and microbiology research. The medium supports the growth of fastidious Lactobacillus species, such as Lactobacillus brevis and Lactobacillus fermentum, and is often used in CO2-enriched atmospheres to enhance growth.

Features and Benefits

NutriSelect® Basic is a powdered culture media offering
  • Quality control with growth promotion test according to industry standards
  • Quality assurance acc. to ISO 9001
  • Cost-efficient powdered media

Preparation Note

Suspend 61.15 g in 1000 ml distilled water containing 1 ml polysorbate 80 (TWEEN® 80; Sigma P8074). Boil to dissolve the medium completely and mix thoroughly. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. If necessary adjust the pH with glacial acetic acid after sterilization.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
TWEEN is a registered trademark of Croda International PLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN8759
BCCN3771
BCCN2036
BCCN1663
BCCM7898

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J.D. DeMan et al.
The Journal of Applied Bacteriology, 23, 130-130 (1960)
Hanan R Shehata et al.
Frontiers in microbiology, 11, 1095-1095 (2020-06-26)
Probiotics are defined as "live microorganisms that, when administered in adequate amounts, confer a health benefit on the host." The diverse health benefits have contributed to rapid increase in probiotic consumption and in the value of probiotic market, valued at
Eleftheria Barouni et al.
Food chemistry, 200, 76-82 (2016-02-03)
The object of the current study was to develop a new process for continuous Feta-type cheese production using a biocatalyst consisting of immobilized rennin on a tubular cellulose/starch gel (TC/SG) composite, which has been proven to be an appropriate carrier
Rupal Mistry et al.
Genetics, 206(2), 889-904 (2017-04-18)
Resident gut bacteria are constantly influencing the immune system, yet the role of the immune system in shaping microbiota composition during an organism's life span has remained unclear. Experiments in mice have been inconclusive due to differences in husbandry schemes
Marie-Paule Nawrot-Esposito et al.
Insects, 11(10) (2020-10-18)
Bioinsecticides made from the bacterium Bacillus thuringiensis (Bt) are the bestselling bioinsecticide worldwide. Among Bt bioinsecticides, those based on the strain Bt subsp. kurstaki (Btk) are widely used in farming to specifically control pest lepidopteran larvae. Although there is much

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