Merck
CN
All Photos(2)

Documents

Safety Information

70138

Millipore

Brain Heart Infusion Agar

suitable for microbiology, NutriSelect® Plus, For the cultivation of fastidious, pathogenic bacteria, yeasts, and molds

Sign Into View Organizational & Contract Pricing
All Photos(2)
Synonym(s):
BHI Agar
NACRES:
NA.74

Quality Level

200

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
brain extract, 7.8 g/L
dextrose, 2.0 g/L
disodium phosphate, 2.5 g/L
heart extract, 9.7 g/L
proteose peptone, 10.0 g/L
sodium chloride, 5.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

pH

7.2-7.6 (25 °C)

application(s)

clinical testing
environmental
food and beverages
life science and biopharma
water monitoring
microbiology

storage temp.

10-30°C

suitability

Ajellomyces spp.
Blastomyces spp.
Enterococcus spp.
Erysipelothrix spp.
Haemophilus spp.
Pneumococcus spp.
nonselective for Candida spp.
nonselective for Clostridium spp.
nonselective for Escherichia coli
nonselective for Lactobacillus spp.
nonselective for Neisseria spp.
nonselective for Shigella spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

Related Categories

General description

Rosenow devised the original medium by adding brain tissue to dextrose agar. These media are nutritious and well buffered to support the growth of a wide range of microorganisms such as Streptococci, Pneumococci, Meningococci, etc. Addition of ascites permits the cultivation of Gonococci. With the addition of 10% defibrinated sheep blood, it is useful for isolation and cultivation of Histoplasma capsulatum and other pathogenic fungi. This medium is to be used for the selective isolation of fastidious fungi (especially of Histoplasma capsulatum and Blastomyces), the growth of the accompanying bacterial and saprophytic yeasts and molds can be almost completely suppressed by adding the following recommended components mixtures: 12 mg Penicillin and 40 ug Streptomycin per liter; 50 mg Chloramphenicol and 500mg Cycloheximide per liter; 50 mg Gentamicin and/or 50 mg Chloramphenicol per liter.
This medium is less suited for identifying haemolytic forms when blood is added due to its glucose content. While handling Histoplasma capsulatum extreme care should be taken to avoid dissemination of its infective spores. The culture should be examined in a closed filtered air cabinet.

Application

BHI agar is a general-purpose medium used for primary isolation of pathogenic aerobic bacteria from clinical specimens and for the cultivation and maintenance of fastidious microorganisms from food, animal feed, water, and other samples
Defibrinated sheep blood added to the basal medium provides essential growth factors for the more fastidious fungal organisms.

Preparation Note

Suspend 52 g in 1 litre of distilled water. Boil to dissolve the medium completely. Distribute into tubes or flasks and sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Selection for vancomycin resistance in clinical isolates of Staph. haemolyticus

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物来源培养基

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

R S Schwalbe et al.
The Journal of infectious diseases, 161(1), 45-51 (1990-01-01)
Killing curves were used to characterize Staphylococcus haemolyticus isolates previously reported to contain subpopulations showing increased resistance to vancomycin. Results suggested that vancomycin and teicoplanin were ineffective at a concentration of 8 micrograms/ml and growth was seen between 24 and
Monica Gupta et al.
Nature communications, 6, 6379-6379 (2015-03-17)
Autophagy, activated by many stresses, plays a critical role in innate immune responses. Here we show that interferon regulatory factor 8 (IRF8) is required for the expression of autophagy-related genes in dendritic cells. Furthermore in macrophages, IRF8 is induced by
Marie-Paule Nawrot-Esposito et al.
Insects, 11(10) (2020-10-18)
Bioinsecticides made from the bacterium Bacillus thuringiensis (Bt) are the bestselling bioinsecticide worldwide. Among Bt bioinsecticides, those based on the strain Bt subsp. kurstaki (Btk) are widely used in farming to specifically control pest lepidopteran larvae. Although there is much
Bettina Schmid et al.
Biomedicines, 8(7) (2020-07-08)
Recently, the role of infection of the intervertebral disc (IVD) with Cutibacterium acnes (C. acnes) as a contributor to disc-related low back pain (LBP) has been discussed. The aim of this study was to investigate whether and how C. acnes
Juan Tur et al.
Cell reports, 32(8), 108079-108079 (2020-08-28)
Mitofusin 2 (Mfn2) plays a major role in mitochondrial fusion and in the maintenance of mitochondria-endoplasmic reticulum contact sites. Given that macrophages play a major role in inflammation, we studied the contribution of Mfn2 to the activity of these cells.

Articles

Clostridia Diagnostic

Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism.

Streptococci - Overview of Detection, Identification, Differentiation and Cultivation Techniques

Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service