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Merck
CN

70149

Nutrient Broth

No. 3, NutriSelect® Plus, powder, for bacteria, pack of 500 g, suitable for microbiology

Synonym(s):

Standard - Nutrient Broth

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.74
MDL number:
eCl@ss:
32039090
Manufacturer/tradename:
NutriSelect® Plus
Form:
powder
Suitability:
bacteria (general purpose media), nonselective for Escherichia coli, nonselective for Pseudomonas spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for coliforms
Application(s):
agriculture
clinical testing
environmental
food and beverages
life science and biopharma
water monitoring, microbiology
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Quality Segment

sterility

non-sterile

product line

NutriSelect® Plus

form

powder

shelf life

limited shelf life, expiry date on the label

composition

meat extract, 1 g/L , peptone, 5 g/L , sodium chloride, 5 g/L , yeast extract, 2 g/L

packaging

pack of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

color

beige

final pH

7.4±0.2 (25 °C)

application(s)

agriculture
clinical testing
environmental
food and beverages
life science and biopharma
water monitoring, microbiology

suitability

bacteria (general purpose media), nonselective for Escherichia coli, nonselective for Pseudomonas spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for coliforms

General description

Nutrient Broth serves as a general-purpose medium designed for the cultivation of microorganisms with minimal nutritional demands. Its nutrient composition, derived from meat infusion and peptone, forms the basis of many culture media. Nutrient Broth No. 3 is a fundamental medium utilized for maintaining microbial cultures and verifying purity. It is particularly effective for cultivating and enumerating non-fastidious bacteria. In its semisolid form, it is ideal for preserving or controlling standard microbial strains. By incorporating biological fluids such as horse or sheep blood, serum, or egg yolk, the medium can be adapted to support the growth of fastidious microorganisms.

Application

Nutrient Broth No. 3 is a liquid medium which serves as base for the culture and accumulation of microorganisms.

Features and Benefits

Features:
  • High-Quality Standards: NutriSelect® Plus powdered culture media are made from carefully selected raw materials, ensuring consistent quality.
  • Wide Range of Applications: Suitable for various microbiological needs, including academic research, food and beverage testing, and pharmaceutical applications.
  • Regulatory Compliance: Products meet industry standards and are compliant with ISO 9001 quality assurance.
  • Innovative Formulations: Includes novel media types such as non-animal-derived alternatives, synthetically defined media, and chromogenic media.
Benefits:
  • Convenience and Safety: Easy-to-use powdered format with minimal clumping and excellent solubility, even in warm or humid conditions.
  • Sustainability: Reduced risks associated with fine dust and toxic substance inhalation, ensuring a safer work environment.
  • Extended Shelf Life: Products have a prolonged shelf life of up to five years.
  • High Reproducibility: Ensures consistent results across batches, exceeding regulatory demands.
  • Comprehensive Portfolio: A vast selection of over 350 products tailored to meet diverse microbiological testing needs.

Preparation Note

Add 13 g to 1 litre of distilled water. Mix well and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany


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Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)



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