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70152

Millipore

Plate Count Agar

NutriSelect® Plus, according to ISO 4833-1:, ISO 17410, GB 4789, powder, for bacteria, pack of 500 g, pack of 2.5 kg

Synonym(s):

Casein-peptone Dextrose Yeast Agar, Standard Methods Agar, Tryptone Glucose Yeast Extract Agar

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About This Item

UNSPSC Code:
41171605
NACRES:
NA.74

Agency

GB 4789
ISO 17410
according to ISO 4833-1:

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 9.0 g/L
dextrose, 1.0 g/L
tryptone, 5.0 g/L
yeast extract, 2.5 g/L

packaging

pack of 2.5 kg
pack of 500 g

manufacturer/tradename

NutriSelect® Plus

storage condition

(Dry place, tightly sealed containers)

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
microbiology
water monitoring

storage temp.

2-25°C

suitability

bacteria
nonselective for Bacillus spp.
nonselective for Escherichia coli
nonselective for Lactobacillus spp.
nonselective for Lactococcus spp.
nonselective for Listeria spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

General description

Plate Count Agar is the recommended medium forthe isolation of microorganisms in milk and other dairy products. The enzymaticdigest of casein (tryptone) with a high level of free amino acids is a nitrogensource. Yeast extract primarily supplies the B-complex vitamins. Glucose is anenergy source for the growth of bacteria, while agar is the solidifying agent.Plate Count Agar is also suitable for determining bacterial count from sterilerooms.

Application

Plate Count Agar is a non-selective medium for the plate count of microorganisms in water, food, milk, dairy products, beverages, animal feed, and other materials.

Preparation Note

Suspend 17.5 g in 1 litre of distilled water. Dissolve by bringing to the boil with frequent stirring, mix and distribute into final containers. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Comparison of β-glucuronidase and indole-based direct plating methods for enumeration of unstressed E. coli

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCL9618
BCCL1863
BCCK1795
BCCG9785
BCCJ5243

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