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Merck
CN

70184

Antibiotic Broth

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Antibiotic Medium No. 3, Assay Broth, Penassay Broth, Grove and Randall Antibiotic Broth

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171504
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sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

dextrose, 1.0 g/L , dipotassium hydrogen phosphate, 3.68 g/L , meat extract, 1.5 g/L , peptone (from meat), 5.0 g/L , potassium dihydrogen phosphate, 1.32 g/L , sodium chloride, 3.5 g/L , yeast extract, 1.5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | susceptibility testing: suitable, microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

clinical testing
food and beverages
life science and biopharma
pharmaceutical, microbiology

suitability

bacteria, nonselective for Escherichia coli, nonselective for Klebsiella spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for coliforms

Quality Level

Application

Used in antibiotic assay work (USP, FDA, Ph Eur) according to Grove & Randall; used in the turbidimetric assay of e.g. penicillin, bacitracin and kanamycin with S. aureus and for the cylinder assay of e.g. erythromycin and chloramphenicol with M. inteus.

Preparation Note

Dissolve 17.5 g in 1 litre of warm (60 C) distilled water and sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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D.C. Grove et al.
Assay Methods of Antibiotics, Med. Encycl. (1955)

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