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Merck
CN

70192

Mueller Hinton Broth

NutriSelect® Plus, according to FDA, NCCLS, powder, for bacteria, pack of 100 g, pack of 500 g

Synonym(s):

MH Broth; Antibacterial potency testing media; Antimicrobial susceptibility testing media

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171504

Key Documents

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Product Name

Mueller Hinton Broth, NutriSelect® Plus, according to FDA, NCCLS, powder, for bacteria, pack of 100 g, pack of 500 g

agency

NCCLS

reg. compliance

according to FDA

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

beef infusion solids, 2.0 g/L
casein hydrolysate, 17.5 g/L
starch, 1.5 g/L

packaging

pack of 100 g
pack of 500 g

manufacturer/tradename

NutriSelect® Plus

storage condition

protect from light

technique(s)

microbe id | susceptibility testing: suitable
microbiological culture: suitable

final pH

7.4±0.2 (25 °C)

application(s)

clinical testing
food and beverages
microbiology

storage temp.

15-25°C

suitability

bacteria

Quality Level

200

Application

Mueller Hinton broth is primarily used to determine the MIC (minimum inhibitory concentration) for several antimicrobial agents. It can also be used to cultivate fastidious organisms like Meningococcus and Gonococcus

General description

Mueller Hinton Broth is used for determining minimal inhibitory concentrations (MICs). Mueller Hinton medium is recommended by FDA, World Health Organization, and NCCLS for testing the most encountered aerobic and facultative anaerobic bacteria in food and clinical material. The medium shows good batch-to-batch reproducibility, it is low in sulfonamide, trimethoprim, and tetracycline inhibitors and yields satisfactory growth of most non-fastidious pathogens.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Preparation Note

Dissolve 21 g in 1 litre of distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

监管及禁止进口产品
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Certificates of Analysis (COA)

Lot/Batch Number
BCCN1507
BCCM3614
BCCL7816
BCCK8309
BCCJ6982

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Branko Jovčić et al.
Antibiotics (Basel, Switzerland), 9(12) (2020-12-17)
The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven
J.H. Muller et al.
Proceedings of the Society For Experimental Biology and Medicine. Society For Experimental Biology and Medicine, 48, 330-330 (1941)
Dennis Wilkens Juhl et al.
Frontiers in cellular and infection microbiology, 10, 526459-526459 (2020-10-27)
Magainin 2 and PGLa are antimicrobial peptides found together in frog skin secretions. When added as a mixture they show an order of magnitude increase in antibacterial activity and in model membrane permeation assays. Here we demonstrate that both peptides
Tracy Lackraj et al.
Journal of bacteriology, 202(11) (2020-04-02)
Dps, a DNA-binding protein from starved cells in Escherichia coli, is part of the bacterial defense system that protects DNA against various cellular stresses. Our lab previously demonstrated that a novel antimicrobial peptide, WRWYCR, enhances acid-induced killing of enterohemorrhagic Escherichia
Konrad Gwozdzinski et al.
Journal of clinical microbiology, 56(5) (2018-03-09)
The plasmid-located colistin resistance gene mcr-1 confers low-level resistance to colistin, a last-line antibiotic against multidrug-resistant Gram-negative bacteria. Current CLSI-EUCAST recommendations require the use of a broth microdilution (BMD) method with cation-adjusted Mueller-Hinton (CA-MH) medium for colistin susceptibility testing, but
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