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70194

Tryptone Water

suitable for microbiology, NutriSelect® Plus

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About This Item

NACRES:
NA.74
UNSPSC Code:
41171607
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sterility

non-sterile

Quality Level

200

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

sodium chloride, 5.0 g/L , tryptone (tryptic hydrolysate of casein), 10.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | specific enzyme detection: suitable, microbiological culture: suitable

final pH

7.5±0.2 (25 °C)

application(s)

environmental
food and beverages
sample preparation
water monitoring, microbiology

suitability

nonselective for Enterobacter spp., nonselective for Escherichia coli, nonselective for Proteus spp., nonselective for Salmonella spp., nonselective for Staphylococcus spp., nonselective for bacteria (General Media), nonselective for coliforms, nonselective for enterobacteriaceae

Application

Tryptone water is primarily used for the cultivation and maintenance of fastidious aerobic and facultative microorganisms such as Escherichia coli and Pseudomonas species.
A liquid medium for the detection of indole-forming microorganisms due to its high content of tryptophan, it is more reliable than peptone water for this purpose.

Preparation Note

Add 15 g to 1 litre of distilled water. Mix well, distribute into final containers and sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Direct plating method for enumeration of E. coli in water
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动物来源培养基

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Certificates of Analysis (COA)

Lot/Batch Number
BCCP3082
BCCM1904
BCCK8192
BCCG5252
BCCB4467

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Handbook of Microbiological Media (2004)
A H Havelaar et al.
The Journal of applied bacteriology, 64(1), 89-98 (1988-01-01)
The direct plating (DP) method for enumerating Escherichia coli in food was adapted for water analysis by membrane filtration and a standardized protocol was described. The DP method was found to give equal or better recoveries of E. coli than

Global Trade Item Number

SKUGTIN
70194-500G04061832820323