70722
E. coli ChromoSelect Agar B
NutriSelect® Plus, suitable for microbiology
Synonym(s):
E. coli Agar B ChromoSelect
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About This Item
NACRES:
NA.85
UNSPSC Code:
41171606
sterility
non-sterile
Quality Segment
form
powder
shelf life
limited shelf life, expiry date on the label
composition
agar, 15 g/L , bile salt mixture, 1.5 g/L , casein enzymic hydrolysate, 20 g/L , X-glucuronide, 0.075 g/L
manufacturer/tradename
NutriSelect® Plus
final pH
7.2±0.2 (25 °C)
application(s)
agriculture
bioburden testing
environmental
food and beverages
water monitoring, microbiology
suitability
selective and differential for Escherichia coli, selective and differential for Klebsiella spp., selective and differential for coliforms
Application
E. coli ChromoSelect Agar B is recommended for the detection and enumeration of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. Most of the Escherichia coli strains can be differentiated from other coliforms by the presence of enzyme glucuronidase which is highly specific for Escherichia coli. Escherichia coli cells absorb X-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives the blue coloration of the colonies.
Preparation Note
Suspend 36.6 g per litre in distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour into sterile petri plates.
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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