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Merck
CN

70722

Millipore

E. coli ChromoSelect Agar B

NutriSelect® Plus, suitable for microbiology

Synonym(s):

E. coli Agar B ChromoSelect

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

Key Documents

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sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
bile salt mixture, 1.5 g/L
casein enzymic hydrolysate, 20 g/L
X-glucuronide, 0.075 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

7.2±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

suitability

selective and differential for Escherichia coli
selective and differential for Klebsiella spp.
selective and differential for coliforms

Related Categories

Application

E. coli ChromoSelect Agar B is recommended for the detection and enumeration of Escherichia coli in foods without further confirmation on membrane filter or by indole reagent. Most of the Escherichia coli strains can be differentiated from other coliforms by the presence of enzyme glucuronidase which is highly specific for Escherichia coli. Escherichia coli cells absorb X-glucuronide and the intracellular glucuronidase splits the bond between the chromophore and the glucuronide. The released chromophore gives the blue coloration of the colonies.

Preparation Note

Suspend 36.6 g per litre in distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cool to 50°C and pour into sterile petri plates.

Other Notes

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物来源培养基
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Certificates of Analysis (COA)

Lot/Batch Number
BCCL1565
BCCG9935
BCCD8519
BCCC1970
BCCB3417

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Articles

Differentiation and Detection of Escherichia coli, Coliforms and Enterobacteriaceae

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

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选择性培养基可以为微生物的检测、识别和计数提供更快的结果和肉眼确认

用于分化和检测大肠杆菌和其他大肠菌群的选择性生长培养基

有关用于分化和检测大肠杆菌和其他大肠菌群的选择性生长培养基的一篇文章。

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