71013BC
GDS Top 6 STEC (eae) Tq
BioControl, Molecular based PCR test for the detection of the eae gene in "Top Six" non-O157 Shiga Toxigenic E. coli (STEC) in food
shelf life
6‑12 mo.
manufacturer/tradename
BioControl Systems 71013-100
application(s)
food and beverages
compatibility
BioControl GDS
storage temp.
2-8°C
General description
GDS Top 6 STEC Tq is an automated nucleic acid amplification system for the detection of the eae gene in the following select O-serogroups of E. coli: O103, O111, O121, O145, O26 and O45. Assurance GDS utilizes a proprietary IMS-based sample preparation procedure to isolate organisms belonging to these specific 6 O-serogroups prior to genetic analysis for the presence of the eae gene.
Application
PCR, Immunomagnetic Separation (IMS)
Features and Benefits
- Sequential approach to detecting Top STEC. Following sample preparation with IMS against the Top 6 O groups, samples are analyzed for the presence of (eae) with the GDS Top 6 STEC (eae) assay. Positive samples are subsequently analyzed for Shiga Toxin genes with the GDS Shiga Toxin Genes (Top 6) assay.
- Single-step 10-18 h enrichment in mEHEC, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC
Other Notes
- Top STEC (eae) Amplification Tubes Tq
- Top 6 STEC Concentration Reagent
- Resuspension Buffer Tq
- Top STEC Wash Solution
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