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GDS MPX Top 6 STEC

BioControl, Molecular based PCR test for the detection of the "Top Six" non-O157 Shiga Toxigenic E. coli (STEC) in food

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About This Item

NACRES:
NB.61
UNSPSC Code:
41106302
Technical Service
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Quality Level

200

shelf life

6‑12 mo.

manufacturer/tradename

BioControl Systems 71016-100

storage temp.

2-8°C

General description

GDS MPX Top 6 STEC is an automated nucleic acid amplification system for the detection of the “Top Six” non-O157 Shiga Toxigenic E. coli (STEC) in raw ground beef, raw beef trim, raw spinach, and raw mixed greens (baby lettuce and other young leafy green vegetables). The "Top Six" non-O157 STEC are defined as E. coli belonging to serogroups O103, O111, O121, O145, O26 or O45 that possess both the eae gene and at least one of the Shiga Toxin Genes stx1 or stx2. GDS MPX Top 6 STEC utilizes a proprietary IMS-based sample preparation procedure to isolate organisms belonging to 6 specific Top STEC O-serogroups (O103, O111, O121, O145, O26 and O45) prior to genetic analysis for the associated pathogenicity genes.

Application

PCR, Immunomagnetic Separation (IMS)

Features and Benefits

  • Following sample preparation with IMS against the Top 6 O groups, samples are analyzed with a single multiplex assay for the presence of (eae), stx1, and stx2
  • Single-step 10-18 h enrichment in mEHEC®, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC

Other Notes

  • MPX Top STEC Amplification Tubes
  • Top 6 STEC Concentration Reagent
  • Resuspension Buffer Tq
  • Top STEC Wash Solution

Legal Information

mEHEC is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Corrosion
GHS05

flash_point_f

Not applicable

flash_point_c

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signalword

Danger

Hazard Classifications

ED ENV 1 - Met. Corr. 1

Storage Class

10 - Combustible liquids

Regulatory Information

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