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Merck
CN

71017BC

Assurance® GDS Top 7 STEC (eae) Tq Kit

suitable for PCR testing in Food and Beverages applications

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About This Item

UNSPSC Code:
41106302
NACRES:
NA.85
Agency:
AOAC® (PTM)
Suitability:
enterovirulent E. coli (EEC) (shiga toxigenic)
Manufacturer/tradename:
Assurance GDS®, BioControl Systems 71017-100
Application(s):
food and beverages
pathogen testing
Technique(s):
PCR: suitable
Storage temp.:
2-8°C
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Product Name

GDS Top 7 STEC (eae) Tq, BioControl, Molecular based PCR test for the detection of the eae gene in "Top Seven" Shiga Toxigenic E. coli (STEC) in food

agency

AOAC® (PTM)

Quality Segment

shelf life

6‑12 mo.

packaging

pkg of 100 tests

manufacturer/tradename

Assurance GDS®, BioControl Systems 71017-100

technique(s)

PCR: suitable

application(s)

food and beverages
pathogen testing

compatibility

for use with GDS Rotor-Gene

storage temp.

2-8°C

suitability

enterovirulent E. coli (EEC) (shiga toxigenic)

General description

Assurance® GDS is an advanced genetic detection system for food pathogens. The Top 7 STEC (eae) Tq is an automated nucleic acid amplification system for the detection of the eae gene in the following Top 7 O serogroups of E. coli: O103, O111, O121, O145, O26, O45, and O157. Assurance® GDS utilizes a proprietary PickPen® IMS-based sample preparation procedure to quickly and effectively capture organisms belonging to the 7 specific Top STEC O serogroups prior to genetic analysis for the presence of the eae gene.

Application

Assurance® GDS Top 7 STEC (eae) Tq is designed for use in conjunction with Assurance® GDS Shiga Toxin Genes (Top 7) Tq to detect the presence of Shiga Toxigenic E. coli (STEC) in foods, ingredients, and environmental samples.
PCR, Immunomagnetic Separation (IMS)

Features and Benefits

  • Sequential approach to detecting Top STEC. Following sample preparation with IMS against the Top 7 O groups, samples are analyzed for the presence of (eae) with the GDS Top 7 STEC (eae) assay. Positive samples are subsequently analyzed for Shiga Toxin genes with the Assurance® GDS Shiga Toxin Genes Top 7 assay.
  • Top 7 STEC (includes E. coli O157:H7)
  • Single-step 10-18 h enrichment in mEHEC, enrichment media common to all BioControl methods for E.coli O157:H7, Shiga Toxin genes, and Top STEC

Other Notes

  • Top STEC (eae) Amplification Tubes Tq
  • Top 7 STEC Concentration Reagent
  • Resuspension Buffer Tq
  • Top STEC Wash Solution

Legal Information

AOAC is a registered trademark of AOAC International
Assurance is a registered trademark of Merck KGaA, Darmstadt, Germany
Assurance GDS is a registered trademark of BioControl Systems, Inc.
PICKPEN is a registered trademark of Merck KGaA, Darmstadt, Germany


pictograms

Corrosion

signalword

Danger

Hazard Classifications

ED ENV 1 - Met. Corr. 1

Storage Class

10 - Combustible liquids



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