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75315

OF Test Nutrient Agar

Name: OF Test Nutrient Agar
Brand: SIAL

suitable for microbiology, NutriSelect^® Basic

Synonym(s):

Oxidative Fermentative Agar acc. to Hugh a. Leifson, OF medium

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About This Item

NACRES:

NA.74

UNSPSC Code:

41171606

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Properties

sterility

non-sterile

Quality Segment

100

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 2.5 g/L , bromothymol blue, 0.08 g/L , casein peptone (pancreatic), 2 g/L , dipotassium hydrogen phosphate, 0.3 g/L , sodium chloride, 5 g/L

manufacturer/tradename

NutriSelect^® Basic

technique(s)

microbe id | utilization test: suitable, microbiological culture: suitable

final pH

7.1±0.2 (25 °C)

antibiotic activity spectrum

Gram-negative bacteria

application(s)

agriculture
clinical testing
environmental
food and beverages, microbiology

suitability

Alcaligenes spp., Chromobacterium spp., Moraxella spp., Pasteurella spp., Acinetobacter spp., Aeromonas spp., flavobacteria, nonselective and differential for Bordetella spp., nonselective and differential for Campylobacter spp., nonselective and differential for Enterococcus spp., nonselective and differential for Escherichia coli, nonselective and differential for Pseudomonas spp., nonselective and differential for Salmonella spp., nonselective and differential for Shigella spp., nonselective and differential for Vibrio spp., nonselective and differential for Yersinia spp., nonselective and differential for bacteria (General Media), nonselective and differential for coliforms, nonselective and differential for enterobacteriaceae

Description

Application

Medium for demonstrating oxidative and fermentative catabolism of carbohydrate acc. to Hugh and Leifson (1953). In particular for the differentiation and classification of gram-negative intestinal bacteria.

Preparation Note

Dissolve 9.88 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Let cool to approx. 50°C and add 100 ml of sterile filtered 10% D(+)-glucose (Cat. No. 49140), lactose (Cat. No. 61340), sucrose (Cat. No. 84100) or other carbohydrate.

Other Notes

Studies on coagulase-positive staphylococci from bovine milk

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product will be discontinued in the near future; however, stock is still available. The recommended replacement product is 1.03865 - OF (Oxidative/Fermentative) medium Base

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c