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75482

Sigma-Aldrich

Gram′s decolorizer solution

for Gram staining

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Synonym(s):
Acetone ethanol mixture
CAS Number:
MDL number:
NACRES:
MA.02

Quality Level

form

liquid

technique(s)

microbe id | staining: suitable

refractive index

n20/D 1.361

density

0.80 g/mL at 20 °C

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

General description

Gram staining is one of the most significant staining techniques in microbiology. Gram′s decolorizer solution is a mixture of ethanol and acetone.

Application

Gram′s decolorizer solution is used in Gram staining procedure, after the iodine step to remove the crystal violet dye. The decolorizer dissolves the lipid layer from the gram-negative cells.

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Central nervous system

Supplementary Hazards

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

68.0 °F

Flash Point(C)

20 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品
易制毒化学品(3类)

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Articles

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For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.

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